Seroatlas · Human Serome Atlas

NKRF

NF-kappa-B-repressing factor

Also known as: ITBA4, NKRF_HUMAN, NRF

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O15226
Gene: NKRF
Ensembl: ENSG00000186416
Chromosome: X
Canonical length: 690 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli

OverviewNCBI Gene

This gene encodes a transcriptional repressor that interacts with specific negative regulatory elements to mediate transcriptional repression of certain nuclear factor kappa B responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Canonical amino-acid sequenceUniProt

690 residues, UniProt reviewed canonical sequence.

>O15226|NKRF
     1  MEKILQMAEG IDIGEMPSYD LVLSKPSKGQ KRHLSTCDGQ NPPKKQAGSK FHARPRFEPV
    61  HFVASSSKDE RQEDPYGPQT KEVNEQTHFA SMPRDIYQDY TQDSFSIQDG NSQYCDSSGF
   121  ILTKDQPVTA NMYFDSGNPA PSTTSQQANS QSTPEPSPSQ TFPESVVAEK QYFIEKLTAT
   181  IWKNLSNPEM TSGSDKINYT YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY
   241  ACEVRCQNIY LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHT FGEDLVVCQI
   301  GMSSYEFPPA LKPPEDLVVL GKDASGQPIF NASAKHWTNF VITENANDAI GILNNSASFN
   361  KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA AADEALKILQ KTQPTYPSVK
   421  SSQCHTGSSP RGSGKKKDIK DLVVYENSSN PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC
   481  KVILESEVIA EAVGVKKTVK YEAAGEAVKT LKKTQPTVIN NLKKGAVEDV ISRNEIQGRS
   541  AEEAYKQQIK EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK
   601  IAKRDIEQII RNYARSESHT DLTFSRELTN DERKQIHQIA QKYGLKSKSH GVGHDRYLVV
   661  GRKRRKEDLL DQLKQEGQVG HYELVMPQAN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NKRF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.45
Highest tissue expression: 12 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 12 nTPM
hypothalamus: 11 nTPM
thymus: 9.4 nTPM
lymph node: 9 nTPM
cerebellum: 8.6 nTPM
tonsil: 8.6 nTPM

Single-cell type

cone photoreceptor cells: 41 nCPM
other brain neurons: 29 nCPM
cdc: 29 nCPM
monocytes: 26 nCPM
t-cells: 26 nCPM
plasma cells: 26 nCPM

Immune cell

naive CD4 T-cell: 2.1 nTPM
T-reg: 2 nTPM
naive CD8 T-cell: 1.9 nTPM
memory B-cell: 1.5 nTPM
memory CD8 T-cell: 1.5 nTPM
basophil: 1.4 nTPM

Brain region

hypothalamus: 26 nTPM
cerebral cortex: 25 nTPM
basal ganglia: 23 nTPM
pons: 22 nTPM
thalamus: 21 nTPM
white matter: 20 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.18
gnomAD pLI: 1
gnomAD missense Z: 2.74

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NKRF in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NKRF as an antibody target. Whether an autoantibody or antibody against NKRF could matter depends on whether native NKRF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NKRF is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NKRF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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