Seroatlas · Human Serome Atlas

DNAJC2

DnaJ homolog subfamily C member 2

Also known as: DNJC2_HUMAN, MPHOSPH11, MPP11, ZRF1, ZUO1, zuotin

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q99543
Gene: DNAJC2
Ensembl: ENSG00000105821
Chromosome: 7
Canonical length: 621 aa
Protein class: Cancer-related genes, Plasma proteins, Predicted intracellular proteins, Transcription factors
Subcellular location: Cytosol

OverviewNCBI Gene

This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

621 residues, UniProt reviewed canonical sequence.

>Q99543|DNAJC2
     1  MLLLPSAADG RGTAITHALT SASTLCQVEP VGRWFEAFVK RRNRNASASF QELEDKKELS
    61  EESEDEELQL EEFPMLKTLD PKDWKNQDHY AVLGLGHVRY KATQRQIKAA HKAMVLKHHP
   121  DKRKAAGEPI KEGDNDYFTC ITKAYEMLSD PVKRRAFNSV DPTFDNSVPS KSEAKDNFFE
   181  VFTPVFERNS RWSNKKNVPK LGDMNSSFED VDIFYSFWYN FDSWREFSYL DEEEKEKAEC
   241  RDERRWIEKQ NRATRAQRKK EEMNRIRTLV DNAYSCDPRI KKFKEEEKAK KEAEKKAKAE
   301  AKRKEQEAKE KQRQAELEAA RLAKEKEEEE VRQQALLAKK EKDIQKKAIK KERQKLRNSC
   361  KTWNHFSDNE AERVKMMEEV EKLCDRLELA SLQCLNETLT SCTKEVGKAA LEKQIEEINE
   421  QIRKEKEEAE ARMRQASKNT EKSTGGGGNG SKNWSEDDLQ LLIKAVNLFP AGTNSRWEVI
   481  ANYMNIHSSS GVKRTAKDVI GKAKSLQKLD PHQKDDINKK AFDKFKKEHG VVPQADNATP
   541  SERFEGPYTD FTPWTTEEQK LLEQALKTYP VNTPERWEKI AEAVPGRTKK DCMKRYKELV
   601  EMVKAKKAAQ EQVLNASRAK K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAJC2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.4
Highest tissue expression: 40 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 40 nTPM
testis: 29 nTPM
skeletal muscle: 29 nTPM
urinary bladder: 18 nTPM
lymph node: 16 nTPM
liver: 15 nTPM

Single-cell type

late primary spermatocytes: 353 nCPM
early spermatids: 219 nCPM
early primary spermatocytes: 202 nCPM
differentiating spermatogonia: 148 nCPM
cardiomyocytes: 131 nCPM
oocytes: 129 nCPM

Immune cell

basophil: 62 nTPM
NK-cell: 16 nTPM
non-classical monocyte: 15 nTPM
naive B-cell: 14 nTPM
T-reg: 13 nTPM
naive CD4 T-cell: 13 nTPM

Brain region

cerebellum: 19 nTPM
white matter: 17 nTPM
hypothalamus: 17 nTPM
cerebral cortex: 16 nTPM
midbrain: 16 nTPM
basal ganglia: 15 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DNAJC2.

Disease | ImmuneIEDB

Conditions an epitope on DNAJC2 was assayed in.

narcolepsy B cell
multiple sclerosis B cell
peripheral nervous system disease B cell
skin melanoma T cell

ReferencesPubMed · IEDB

Publications for DNAJC2 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

2 publications

Elevated levels of autoantibodies against DNAJC2 in sera of patients with atherosclerotic diseases.
2020 · Heliyon · RCR 0.9 · 17 citations
A Latent Autoantibody Axis Associated with Vascular Vulnerability in Ischemic Stroke: Integrated Statistical and Machine-Learning Analysis.
2026 · Int J Mol Sci

Reference: B cellIEDB

1 publication

Whole-Proteome Peptide Microarrays for Profiling Autoantibody Repertoires within Multiple Sclerosis and Narcolepsy.
2017 · J Proteome Res · RCR 2.2 · 57 citations

Reference: T cellIEDB

1 publication

Sensitive identification of neoantigens and cognate TCRs in human solid tumors.
2022 · Nat Biotechnol · RCR 4.9 · 84 citations

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.29
gnomAD pLI: 0.99
gnomAD missense Z: 1.52
DepMap mean gene effect: -0.11
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 12% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

SANT/Myb domain
DnaJ domain
Homedomain-like superfamily
SANT domain
Myb domain
DnaJ domain, conserved site
Chaperone J-domain superfamily
Zuotin-like, zuotin homology domain
DnaJ domain
Zuotin-like, zuotin homology domain
Myb-like DNA-binding domain
Ribosome-associated complex head domain
Ribosome-associated complex head domain superfamily
J-protein Zuotin/DnaJC2
Ribosome-associated complex head domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DNAJC2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNAJC2 as an antibody target. Whether an autoantibody or antibody against DNAJC2 could matter depends on whether native DNAJC2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNAJC2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNAJC2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...