EEF2

Protein identityUniProt · HPA

UniProt accession

P13639

Gene

EEF2

Ensembl

ENSG00000167658

Chromosome

19

Canonical length

858 aa

Protein class

Cancer-related genes, Disease related genes, FDA approved drug targets, Human disease related genes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytosol

OverviewNCBI Gene

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

858 residues, UniProt reviewed canonical sequence.

>P13639|EEF2
     1  MVNFTVDQIR AIMDKKANIR NMSVIAHVDH GKSTLTDSLV CKAGIIASAR AGETRFTDTR
    61  KDEQERCITI KSTAISLFYE LSENDLNFIK QSKDGAGFLI NLIDSPGHVD FSSEVTAALR
   121  VTDGALVVVD CVSGVCVQTE TVLRQAIAER IKPVLMMNKM DRALLELQLE PEELYQTFQR
   181  IVENVNVIIS TYGEGESGPM GNIMIDPVLG TVGFGSGLHG WAFTLKQFAE MYVAKFAAKG
   241  EGQLGPAERA KKVEDMMKKL WGDRYFDPAN GKFSKSATSP EGKKLPRTFC QLILDPIFKV
   301  FDAIMNFKKE ETAKLIEKLD IKLDSEDKDK EGKPLLKAVM RRWLPAGDAL LQMITIHLPS
   361  PVTAQKYRCE LLYEGPPDDE AAMGIKSCDP KGPLMMYISK MVPTSDKGRF YAFGRVFSGL
   421  VSTGLKVRIM GPNYTPGKKE DLYLKPIQRT ILMMGRYVEP IEDVPCGNIV GLVGVDQFLV
   481  KTGTITTFEH AHNMRVMKFS VSPVVRVAVE AKNPADLPKL VEGLKRLAKS DPMVQCIIEE
   541  SGEHIIAGAG ELHLEICLKD LEEDHACIPI KKSDPVVSYR ETVSEESNVL CLSKSPNKHN
   601  RLYMKARPFP DGLAEDIDKG EVSARQELKQ RARYLAEKYE WDVAEARKIW CFGPDGTGPN
   661  ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV RFDVHDVTLH ADAIHRGGGQ
   721  IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ CPEQVVGGIY GVLNRKRGHV FEESQVAGTP
   781  MFVVKAYLPV NESFGFTADL RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK
   841  RKGLKEGIPA LDNFLDKL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EEF2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.22

Highest tissue expression

3,076 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about EEF2.

Disease | AllUniProt

Conditions EEF2 is implicated in, by any mechanism.

• Spinocerebellar ataxia 26 (SCA26) MIM:609306

Disease | GeneticClinVar

6 pathogenic / likely-pathogenic of 576 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Spinocerebellar ataxia type 26
• EEF2-related neurodevelopmental disorder with multiple anomalies

Disease | ImmuneIEDB

Conditions an epitope on EEF2 was assayed in.

• colon carcinoma T cell
• allergic disease T cell
• lung squamous cell carcinoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.17

gnomAD pLI

1

gnomAD missense Z

4.88

DepMap mean gene effect

-2.35

DepMap dependency class

pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to brain-derived neurotrophic factor stimulus
• glial cell proliferation
• hematopoietic progenitor cell differentiation
• positive regulation of cytoplasmic translation
• positive regulation of translation
• response to endoplasmic reticulum stress
• response to estradiol
• response to ethanol
• response to folic acid
• response to hydrogen peroxide
• response to ischemia
• response to xenobiotic stimulus
• skeletal muscle cell differentiation
• skeletal muscle contraction
• translational elongation
• translation at postsynapse

Molecular functions

• 5S rRNA binding
• actin filament binding
• cadherin binding
• GTP binding
• GTPase activity
• lncRNA binding
• p53 binding
• protein kinase binding
• ribosome binding
• RNA binding
• translation elongation factor activity

Cellular components

• aggresome
• cytoplasm
• cytosol
• extracellular exosome
• extracellular region
• ficolin-1-rich granule lumen
• glutamatergic synapse
• membrane
• nucleus
• plasma membrane
• postsynapse
• ribonucleoprotein complex
• ribosome
• secretory granule lumen

Protein domainsUniProt · Pfam · InterPro

• Elongation factor EFG, domain V-like
• Translational (tr)-type GTP-binding domain
• Translation elongation factor EFTu-like, domain 2
• Small GTP-binding domain
• Translation elongation factor EFG/EF2, domain IV
• Translation protein, beta-barrel domain superfamily
• Small ribosomal subunit protein uS5 domain 2-type fold, subgroup
• Ribosomal protein uS5 domain 2-type superfamily
• P-loop containing nucleoside triphosphate hydrolase
• Tr-type G domain, conserved site
• EF-G domain III/V-like
• Elongation Factor G, domain II
• Elongation factor Tu GTP binding domain
• Elongation factor G C-terminus
• Elongation factor Tu domain 2
• Elongation factor G, domain IV
• Elongation Factor G, domain III

KeywordsUniProt

• Acetylation
• ADP-ribosylation
• Cytoplasm
• Elongation factor
• GTP-binding
• Hydrolase
• Isopeptide bond
• Methylation
• Neurodegeneration
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Protein biosynthesis
• Spinocerebellar ataxia
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of EEF2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HABP4
• RBPMS2
• SERBP1
• HSD17B10
• RPL4