Seroatlas · Human Serome Atlas

PAF1

RNA polymerase II-associated factor 1 homolog

Also known as: F23149_1, FLJ11123, PAF1_HUMAN, PD2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8N7H5
Gene
PAF1
Ensembl
ENSG00000006712
Chromosome
19
Canonical length
531 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli fibrillar center,Vesicles

OverviewNCBI Gene

This gene encodes a subunit of the polymerase associated factor (PAF1) complex. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Canonical amino-acid sequenceUniProt

531 residues, UniProt reviewed canonical sequence.

>Q8N7H5|PAF1
     1  MAPTIQTQAQ REDGHRPNSH RTLPERSGVV CRVKYCNSLP DIPFDPKFIT YPFDQNRFVQ
    61  YKATSLEKQH KHDLLTEPDL GVTIDLINPD TYRIDPNVLL DPADEKLLEE EIQAPTSSKR
   121  SQQHAKVVPW MRKTEYISTE FNRYGISNEK PEVKIGVSVK QQFTEEEIYK DRDSQITAIE
   181  KTFEDAQKSI SQHYSKPRVT PVEVMPVFPD FKMWINPCAQ VIFDSDPAPK DTSGAAALEM
   241  MSQAMIRGMM DEEGNQFVAY FLPVEETLKK RKRDQEEEMD YAPDDVYDYK IAREYNWNVK
   301  NKASKGYEEN YFFIFREGDG VYYNELETRV RLSKRRAKAG VQSGTNALLV VKHRDMNEKE
   361  LEAQEARKAQ LENHEPEEEE EEEMETEEKE AGGSDEEQEK GSSSEKEGSE DEHSGSESER
   421  EEGDRDEASD KSGSGEDESS EDEARAARDK EEIFGSDADS EDDADSDDED RGQAQGGSDN
   481  DSDSGSNGGG QRSRSHSRSA SPFPSGSEHS AQEDGSEAAA SDSSEADSDS D

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PAF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.55
Highest tissue expression
61 nTPM

Expression across tissuesHPA

Tissue

  • testis: 61 nTPM
  • choroid plexus: 60 nTPM
  • skeletal muscle: 56 nTPM
  • spinal cord: 48 nTPM
  • ovary: 46 nTPM
  • liver: 45 nTPM

Single-cell type

  • esophageal apical cells: 157 nCPM
  • late primary spermatocytes: 138 nCPM
  • syncytiotrophoblasts: 81 nCPM
  • endometrial glandular cells: 72 nCPM
  • epicardial cells: 57 nCPM
  • epididymal basal cells: 57 nCPM

Immune cell

  • memory B-cell: 40 nTPM
  • naive B-cell: 37 nTPM
  • eosinophil: 34 nTPM
  • basophil: 32 nTPM
  • neutrophil: 31 nTPM
  • MAIT T-cell: 31 nTPM

Brain region

  • white matter: 50 nTPM
  • hypothalamus: 49 nTPM
  • medulla oblongata: 45 nTPM
  • basal ganglia: 44 nTPM
  • cerebral cortex: 43 nTPM
  • thalamus: 43 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.62
gnomAD pLI
0
gnomAD missense Z
2.75
DepMap mean gene effect
-0.78
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • RNA polymerase II associated factor Paf1
  • Paf1

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PAF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PAF1 as an antibody target. Whether an autoantibody or antibody against PAF1 could matter depends on whether native PAF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PAF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PAF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PAF1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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