Seroatlas · Human Serome Atlas

NELFB

Negative elongation factor B

Also known as: COBRA1, KIAA1182, NELF-B, NELFB_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8WX92
Gene: NELFB
Ensembl: ENSG00000188986
Chromosome: 9
Canonical length: 580 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

580 residues, UniProt reviewed canonical sequence.

>Q8WX92|NELFB
     1  MFAGLQDLGV ANGEDLKETL TNCTEPLKAI EQFQTENGVL LPSLQSALPF LDLHGTPRLE
    61  FHQSVFDELR DKLLERVSAI ASEGKAEERY KKLEDLLEKS FSLVKMPSLQ PVVMCVMKHL
   121  PKVPEKKLKL VMADKELYRA CAVEVKRQIW QDNQALFGDE VSPLLKQYIL EKESALFSTE
   181  LSVLHNFFSP SPKTRRQGEV VQRLTRMVGK NVKLYDMVLQ FLRTLFLRTR NVHYCTLRAE
   241  LLMSLHDLDV GEICTVDPCH KFTWCLDACI RERFVDSKRA RELQGFLDGV KKGQEQVLGD
   301  LSMILCDPFA INTLALSTVR HLQELVGQET LPRDSPDLLL LLRLLALGQG AWDMIDSQVF
   361  KEPKMEVELI TRFLPMLMSF LVDDYTFNVD QKLPAEEKAP VSYPNTLPES FTKFLQEQRM
   421  ACEVGLYYVL HITKQRNKNA LLRLLPGLVE TFGDLAFGDI FLHLLTGNLA LLADEFALED
   481  FCSSLFDGFF LTASPRKENV HRHALRLLIH LHPRVAPSKL EALQKALEPT GQSGEAVKEL
   541  YSQLGEKLEQ LDHRKPSPAQ AAETPALELP LPSVPAPAPL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NELFB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.3
Highest tissue expression: 57 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 57 nTPM
amygdala: 48 nTPM
skeletal muscle: 48 nTPM
skin: 45 nTPM
hippocampal formation: 44 nTPM
heart muscle: 42 nTPM

Single-cell type

breast myoepithelial cells: 80 nCPM
cardiomyocytes: 60 nCPM
breast lactating cells: 49 nCPM
goblet cells: 47 nCPM
esophageal apical cells: 44 nCPM
colonocytes: 42 nCPM

Immune cell

eosinophil: 5.5 nTPM
plasmacytoid DC: 4.7 nTPM
gdT-cell: 4.3 nTPM
T-reg: 4.3 nTPM
NK-cell: 4.1 nTPM
memory CD4 T-cell: 3.6 nTPM

Brain region

medulla oblongata: 62 nTPM
midbrain: 60 nTPM
thalamus: 60 nTPM
amygdala: 58 nTPM
cerebral cortex: 55 nTPM
hippocampal formation: 52 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.6
gnomAD pLI: 0
gnomAD missense Z: 1.66
DepMap mean gene effect: -0.92
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

RNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

Cofactor of BRCA1
Cofactor of BRCA1 (COBRA1)

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NELFB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NELFB as an antibody target. Whether an autoantibody or antibody against NELFB could matter depends on whether native NELFB is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NELFB is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NELFB as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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