NELFE

Protein identityUniProt · HPA

UniProt accession

P18615

Gene

NELFE

Ensembl

ENSG00000204356

Chromosome

6

Canonical length

380 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

380 residues, UniProt reviewed canonical sequence.

>P18615|NELFE
     1  MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL SEQPVMDTAT
    61  ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE KGPVPTFQPF QRSISADDDL
   121  QESSRRPQRK SLYESFVSSS DRLRELGPDG EEAEGPGAGD GPPRSFDWGY EERSGAHSSA
   181  SPPRSRSRDR SHERNRDRDR DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR
   241  DREGPFRRSD SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV
   301  TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV QNSPKGCHRD
   361  KRTQIVYSDD VYKENLVDGF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NELFE can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.57

Highest tissue expression

151 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.56

gnomAD pLI

0.01

gnomAD missense Z

1.57

DepMap mean gene effect

-0.42

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of transcription by RNA polymerase II
• negative regulation of transcription elongation by RNA polymerase II
• positive regulation of ERK1 and ERK2 cascade
• positive regulation of transcription by RNA polymerase II
• transcription pausing by RNA polymerase II

Molecular functions

• chromatin binding
• mRNA binding
• RNA binding

Cellular components

• chromatin
• NELF complex
• nuclear body
• nucleoplasm
• nucleus
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• RNA recognition motif domain
• Nucleotide-binding alpha-beta plait domain superfamily
• RNA-binding domain superfamily
• RNA recognition motif
• Negative elongation factor E
• Negative elongation factor E, RNA recognition motif

KeywordsUniProt

• ADP-ribosylation
• Chromosome
• Coiled coil
• Isopeptide bond
• Nucleus
• Phosphoprotein
• Repeat
• Repressor
• RNA-binding
• Transcription
• Transcription regulation
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of NELFE in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NELFB
• SUPT5H
• SUPT4H1
• NELFA
• YWHAG