Seroatlas · Human Serome Atlas

NFKBIA

NF-kappa-B inhibitor alpha

Also known as: IkappaBalpha, IKBA, IKBA_HUMAN, MAD-3, NFKBI

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P25963
Gene
NFKBIA
Ensembl
ENSG00000100906
Chromosome
14
Canonical length
317 aa
Protein class
Cancer-related genes, Disease related genes, Human disease related genes, Predicted intracellular proteins, Transcription factors
Subcellular location
Cytosol

OverviewNCBI Gene

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Canonical amino-acid sequenceUniProt

317 residues, UniProt reviewed canonical sequence.

>P25963|NFKBIA
     1  MFQAAERPQE WAMEGPRDGL KKERLLDDRH DSGLDSMKDE EYEQMVKELQ EIRLEPQEVP
    61  RGSEPWKQQL TEDGDSFLHL AIIHEEKALT MEVIRQVKGD LAFLNFQNNL QQTPLHLAVI
   121  TNQPEIAEAL LGAGCDPELR DFRGNTPLHL ACEQGCLASV GVLTQSCTTP HLHSILKATN
   181  YNGHTCLHLA SIHGYLGIVE LLVSLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG
   241  ADVNRVTYQG YSPYQLTWGR PSTRIQQQLG QLTLENLQML PESEDEESYD TESEFTEFTE
   301  DELPYDDCVF GGQRLTL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NFKBIA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
961 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 961 nTPM
  • lung: 357 nTPM
  • urinary bladder: 330 nTPM
  • adipose tissue: 270 nTPM
  • skin: 253 nTPM
  • spleen: 219 nTPM

Single-cell type

  • neutrophils: 7,959 nCPM
  • epididymal basal cells: 3,421 nCPM
  • basal keratinocytes: 2,778 nCPM
  • monocytes: 2,714 nCPM
  • mast cells: 2,589 nCPM
  • suprabasal keratinocytes: 2,318 nCPM

Immune cell

  • neutrophil: 749 nTPM
  • total PBMC: 370 nTPM
  • classical monocyte: 347 nTPM
  • myeloid DC: 332 nTPM
  • naive B-cell: 261 nTPM
  • basophil: 257 nTPM

Brain region

  • medulla oblongata: 79 nTPM
  • cerebral cortex: 74 nTPM
  • thalamus: 66 nTPM
  • white matter: 58 nTPM
  • choroid plexus: 57 nTPM
  • pons: 54 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NFKBIA.

Disease | AllUniProt

Conditions NFKBIA is implicated in, by any mechanism.

  • Ectodermal dysplasia and immunodeficiency 2 (EDAID2) MIM:612132

Disease | GeneticClinVar

11 pathogenic / likely-pathogenic of 390 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Ectodermal dysplasia and immunodeficiency 2

Disease | ImmuneIEDB

Conditions an epitope on NFKBIA was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.19
gnomAD pLI
0.99
gnomAD missense Z
0.71
DepMap mean gene effect
-0.01
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NFKBIA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NFKBIA as an antibody target. Whether an autoantibody or antibody against NFKBIA could matter depends on whether native NFKBIA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NFKBIA is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NFKBIA as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NFKBIA. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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