Seroatlas · Human Serome Atlas

E2F1

Transcription factor E2F1

Also known as: E2F1_HUMAN, RBBP3, RBP3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q01094
Gene
E2F1
Ensembl
ENSG00000101412
Chromosome
20
Canonical length
437 aa
Protein class
Cancer-related genes, Plasma proteins, Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm,Centrosome

OverviewNCBI Gene

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

437 residues, UniProt reviewed canonical sequence.

>Q01094|E2F1
     1  MALAGAPAGG PCAPALEALL GAGALRLLDS SQIVIISAAQ DASAPPAPTG PAAPAAGPCD
    61  PDLLLFATPQ APRPTPSAPR PALGRPPVKR RLDLETDHQY LAESSGPARG RGRHPGKGVK
   121  SPGEKSRYET SLNLTTKRFL ELLSHSADGV VDLNWAAEVL KVQKRRIYDI TNVLEGIQLI
   181  AKKSKNHIQW LGSHTTVGVG GRLEGLTQDL RQLQESEQQL DHLMNICTTQ LRLLSEDTDS
   241  QRLAYVTCQD LRSIADPAEQ MVMVIKAPPE TQLQAVDSSE NFQISLKSKQ GPIDVFLCPE
   301  ETVGGISPGK TPSQEVTSEE ENRATDSATI VSPPPSSPPS SLTTDPSQSL LSLEQEPLLS
   361  RMGSLRAPVD EDRLSPLVAA DSLLEHVRED FSGLLPEEFI SLSPPHEALD YHFGLEEGEG
   421  IRDLFDCDFG DLTPLDF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against E2F1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.58
Highest tissue expression
32 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 32 nTPM
  • thymus: 9.9 nTPM
  • testis: 9.3 nTPM
  • lymph node: 8.1 nTPM
  • basal ganglia: 7.3 nTPM
  • hippocampal formation: 6.7 nTPM

Single-cell type

  • platelets: 142 nCPM
  • differentiating spermatogonia: 38 nCPM
  • erythrocyte progenitors: 38 nCPM
  • early primary spermatocytes: 24 nCPM
  • monocyte progenitors: 21 nCPM
  • megakaryocyte progenitors: 20 nCPM

Immune cell

  • non-classical monocyte: 1 nTPM
  • memory B-cell: 0.5 nTPM
  • NK-cell: 0.4 nTPM
  • naive CD8 T-cell: 0.3 nTPM
  • intermediate monocyte: 0.2 nTPM
  • eosinophil: 0.1 nTPM

Brain region

  • white matter: 10 nTPM
  • cerebral cortex: 10 nTPM
  • medulla oblongata: 9 nTPM
  • amygdala: 8.1 nTPM
  • basal ganglia: 7.9 nTPM
  • spinal cord: 7.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.23
gnomAD pLI
0.99
gnomAD missense Z
2.07
DepMap mean gene effect
-0.13
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of E2F1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads E2F1 as an antibody target. Whether an autoantibody or antibody against E2F1 could matter depends on whether native E2F1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

E2F1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label E2F1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/E2F1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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