Seroatlas · Human Serome Atlas

NET1

Neuroepithelial cell-transforming gene 1 protein

Also known as: ARHG8_HUMAN, ARHGEF8, NET1A

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q7Z628
Gene: NET1
Ensembl: ENSG00000173848
Chromosome: 10
Canonical length: 596 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Vesicles

OverviewNCBI Gene

This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Canonical amino-acid sequenceUniProt

596 residues, UniProt reviewed canonical sequence.

>Q7Z628|NET1
     1  MEPELAAQKQ PRPRRRSRRA SGLSTEGATG PSADTSGSEL DGRCSLRRGS SFTFLTPGPN
    61  WDFTLKRKRR EKDDDVVSLS SLDLKEPSNK RVRPLARVTS LANLISPVRN GAVRRFGQTI
   121  QSFTLRGDHR SPASAQKFSS RSTVPTPAKR RSSALWSEML DITMKESLTT REIRRQEAIY
   181  EMSRGEQDLI EDLKLARKAY HDPMLKLSIM SEEELTHIFG DLDSYIPLHE DLLTRIGEAT
   241  KPDGTVEQIG HILVSWLPRL NAYRGYCSNQ LAAKALLDQK KQDPRVQDFL QRCLESPFSR
   301  KLDLWSFLDI PRSRLVKYPL LLKEILKHTP KEHPDVQLLE DAILIIQGVL SDINLKKGES
   361  ECQYYIDKLE YLDEKQRDPR IEASKVLLCH GELRSKSGHK LYIFLFQDIL VLTRPVTRNE
   421  RHSYQVYRQP IPVQELVLED LQDGDVRMGG SFRGAFSNSE KAKNIFRIRF HDPSPAQSHT
   481  LQANDVFHKQ QWFNCIRAAI APFQSAGSPP ELQGLPELHE ECEGNHPSAR KLTAQRRAST
   541  VSSVTQVEVD ENAYRCGSGM QMAEDSKSLK THQTQPGIRR ARDKALSGGK RKETLV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NET1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.45
Highest tissue expression: 89 nTPM

Expression across tissuesHPA

Tissue

blood vessel: 89 nTPM
salivary gland: 88 nTPM
colon: 87 nTPM
rectum: 83 nTPM
stomach: 74 nTPM
small intestine: 70 nTPM

Single-cell type

esophageal apical cells: 777 nCPM
ocular epithelial cells: 425 nCPM
vascular smooth muscle cells: 292 nCPM
lacrimal acinar cells: 290 nCPM
prostatic hillock cells: 280 nCPM
foveolar cells: 275 nCPM

Immune cell

naive CD4 T-cell: 8.7 nTPM
myeloid DC: 4.9 nTPM
T-reg: 4 nTPM
total PBMC: 2.2 nTPM
basophil: 2 nTPM
plasmacytoid DC: 2 nTPM

Brain region

choroid plexus: 79 nTPM
pons: 60 nTPM
medulla oblongata: 55 nTPM
thalamus: 55 nTPM
white matter: 50 nTPM
cerebellum: 48 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.77
gnomAD pLI: 0
gnomAD missense Z: 1.08
DepMap mean gene effect: 0
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

guanyl-nucleotide exchange factor activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NET1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NET1 as an antibody target. Whether an autoantibody or antibody against NET1 could matter depends on whether native NET1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NET1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NET1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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