Seroatlas · Human Serome Atlas

ARHGEF3

Rho guanine nucleotide exchange factor 3

Also known as: ARHG3_HUMAN, DKFZP434F2429, GEF3, STA3, XPLN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NR81
Gene
ARHGEF3
Ensembl
ENSG00000163947
Chromosome
3
Canonical length
526 aa
Protein class
Predicted intracellular proteins
Subcellular location
Cytosol

OverviewNCBI Gene

Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

526 residues, UniProt reviewed canonical sequence.

>Q9NR81|ARHGEF3
     1  MVAKDYPFYL TVKRANCSLE LPPASGPAKD AEEPSNKRVK PLSRVTSLAN LIPPVKATPL
    61  KRFSQTLQRS ISFRSESRPD ILAPRPWSRN AAPSSTKRRD SKLWSETFDV CVNQMLTSKE
   121  IKRQEAIFEL SQGEEDLIED LKLAKKAYHD PMLKLSIMTE QELNQIFGTL DSLIPLHEEL
   181  LSQLRDVRKP DGSTEHVGPI LVGWLPCLSS YDSYCSNQVA AKALLDHKKQ DHRVQDFLQR
   241  CLESPFSRKL DLWNFLDIPR SRLVKYPLLL REILRHTPND NPDQQHLEEA INIIQGIVAE
   301  INTKTGESEC RYYKERLLYL EEGQKDSLID SSRVLCCHGE LKNNRGVKLH VFLFQEVLVI
   361  TRAVTHNEQL CYQLYRQPIP VKDLLLEDLQ DGEVRLGGSL RGAFSNNERI KNFFRVSFKN
   421  GSQSQTHSLQ ANDTFNKQQW LNCIRQAKET VLCAAGQAGV LDSEGSFLNP TTGSRELQGE
   481  TKLEQMDQSD SESDCSMDTS EVSLDCERME QTDSSCGNSR HGESNV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGEF3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
49 nTPM

Expression across tissuesHPA

Tissue

  • parathyroid gland: 49 nTPM
  • adrenal gland: 41 nTPM
  • endometrium: 36 nTPM
  • thymus: 34 nTPM
  • smooth muscle: 32 nTPM
  • choroid plexus: 31 nTPM

Single-cell type

  • podocytes: 1,580 nCPM
  • sertoli cells: 857 nCPM
  • choroid plexus epithelial cells: 754 nCPM
  • distal convoluted tubule cells: 607 nCPM
  • renal connecting tubule cells: 559 nCPM
  • adrenal cortex cells: 500 nCPM

Immune cell

  • gdT-cell: 96 nTPM
  • memory CD8 T-cell: 85 nTPM
  • total PBMC: 76 nTPM
  • memory CD4 T-cell: 74 nTPM
  • naive CD8 T-cell: 74 nTPM
  • MAIT T-cell: 73 nTPM

Brain region

  • choroid plexus: 71 nTPM
  • hippocampal formation: 60 nTPM
  • cerebral cortex: 41 nTPM
  • cerebellum: 35 nTPM
  • basal ganglia: 31 nTPM
  • white matter: 31 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.52
gnomAD pLI
0
gnomAD missense Z
2.08
DepMap mean gene effect
0
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARHGEF3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARHGEF3 as an antibody target. Whether an autoantibody or antibody against ARHGEF3 could matter depends on whether native ARHGEF3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARHGEF3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARHGEF3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ARHGEF3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...