Seroatlas · Human Serome Atlas

MCF2L

Guanine nucleotide exchange factor DBS

Also known as: ARHGEF14, DBS, KIAA0362, MCF2L_HUMAN, OST

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O15068
Gene
MCF2L
Ensembl
ENSG00000126217
Chromosome
13
Canonical length
1137 aa
Protein class
Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Canonical amino-acid sequenceUniProt

1137 residues, UniProt reviewed canonical sequence.

>O15068|MCF2L
     1  MFDCWRFILC KRPGSNSYSS PQRPNEAKKE ETDHQIDVSD VIRLVQDTPE ATAMATDEIM
    61  HQDIVPLCAA DIQDQLKKRF AYLSGGRGQD GSPVITFPDY PAFSEIPDKE FQNVMTYLTS
   121  IPSLQDAGIG FILVIDRRRD KWTSVKASVL RIAASFPANL QLVLVLRPTG FFQRTLSDIA
   181  FKFNRDDFKM KVPVIMLSSV PDLHGYIDKS QLTEDLGGTL DYCHSRWLCQ RTAIESFALM
   241  VKQTAQMLQS FGTELAETEL PNDVQSTSSV LCAHTEKKDK AKEDLRLALK EGHSVLESLR
   301  ELQAEGSEPS VNQDQLDNQA TVQRLLAQLN ETEAAFDEFW AKHQQKLEQC LQLRHFEQGF
   361  REVKAILDAA SQKIATFTDI GNSLAHVEHL LRDLASFEEK SGVAVERARA LSLDGEQLIG
   421  NKHYAVDSIR PKCQELRHLC DQFSAEIARR RGLLSKSLEL HRRLETSMKW CDEGIYLLAS
   481  QPVDKCQSQD GAEAALQEIE KFLETGAENK IQELNAIYKE YESILNQDLM EHVRKVFQKQ
   541  ASMEEVFHRR QASLKKLAAR QTRPVQPVAP RPEALAKSPC PSPGIRRGSE NSSSEGGALR
   601  RGPYRRAKSE MSESRQGRGS AGEEEESLAI LRRHVMSELL DTERAYVEEL LCVLEGYAAE
   661  MDNPLMAHLL STGLHNKKDV LFGNMEEIYH FHNRIFLREL ENYTDCPELV GRCFLERMED
   721  FQIYEKYCQN KPRSESLWRQ CSDCPFFQEC QRKLDHKLSL DSYLLKPVQR ITKYQLLLKE
   781  MLKYSRNCEG AEDLQEALSS ILGILKAVND SMHLIAITGY DGNLGDLGKL LMQGSFSVWT
   841  DHKRGHTKVK ELARFKPMQR HLFLHEKAVL FCKKREENGE GYEKAPSYSY KQSLNMAAVG
   901  ITENVKGDAK KFEIWYNARE EVYIVQAPTP EIKAAWVNEI RKVLTSQLQA CREASQHRAL
   961  EQSQSLPLPA PTSTSPSRGN SRNIKKLEER KTDPLSLEGY VSSAPLTKPP EKGKGWSKTS
  1021  HSLEAPEDDG GWSSAEEQIN SSDAEEDGGL GPKKLVPGKY TVVADHEKGG PDALRVRSGD
  1081  VVELVQEGDE GLWYVRDPTT GKEGWVPASS LSVRLGPSGS AQCLSSSGKA HVPRAHP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MCF2L can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
158 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 158 nTPM
  • cerebral cortex: 73 nTPM
  • heart muscle: 51 nTPM
  • pituitary gland: 43 nTPM
  • adipose tissue: 42 nTPM
  • breast: 39 nTPM

Single-cell type

  • cone photoreceptor cells: 499 nCPM
  • proximal tubule cells: 383 nCPM
  • microglia: 377 nCPM
  • somatotrophs: 278 nCPM
  • lactotrophs: 254 nCPM
  • gonadotrophs: 251 nCPM

Immune cell

  • naive B-cell: 0.4 nTPM
  • naive CD4 T-cell: 0.4 nTPM
  • total PBMC: 0.4 nTPM
  • neutrophil: 0.3 nTPM
  • gdT-cell: 0.1 nTPM
  • MAIT T-cell: 0.1 nTPM

Brain region

  • cerebellum: 339 nTPM
  • cerebral cortex: 214 nTPM
  • pons: 190 nTPM
  • medulla oblongata: 178 nTPM
  • thalamus: 176 nTPM
  • white matter: 158 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.5
gnomAD pLI
0
gnomAD missense Z
1.85
DepMap mean gene effect
-0.07
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MCF2L in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MCF2L as an antibody target. Whether an autoantibody or antibody against MCF2L could matter depends on whether native MCF2L is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MCF2L is annotated at the cell surface, where native MCF2L is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label MCF2L as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MCF2L. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...