Seroatlas · Human Serome Atlas

ARHGEF25

Rho guanine nucleotide exchange factor 25

Also known as: ARHGP_HUMAN, GEFT, p63RhoGEF

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q86VW2
Gene: ARHGEF25
Ensembl: ENSG00000240771
Chromosome: 12
Canonical length: 580 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Rho GTPases alternate between an inactive GDP-bound state and an active GTP-bound state, and GEFs facilitate GDP/GTP exchange. This gene encodes a guanine nucleotide exchange factor (GEF) which interacts with Rho GTPases involved in contraction of vascular smooth muscles, regulation of responses to angiotensin II and lens cell differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Canonical amino-acid sequenceUniProt

580 residues, UniProt reviewed canonical sequence.

>Q86VW2|ARHGEF25
     1  MRGGHKGGRC ACPRVIRKVL AKCGCCFARG GRESYSIAGS EGSISASAAS GLAAPSGPSS
    61  GLSSGPCSPG PPGPVSGLRR WLDHSKHCLS VETEADSGQA GPYENWMLEP ALATGEELPE
   121  LTLLTTLLEG PGDKTQPPEE ETLSQAPESE EEQKKKALER SMYVLSELVE TEKMYVDDLG
   181  QIVEGYMATM AAQGVPESLR GRDRIVFGNI QQIYEWHRDY FLQELQRCLK DPDWLAQLFI
   241  KHERRLHMYV VYCQNKPKSE HVVSEFGDSY FEELRQQLGH RLQLNDLLIK PVQRIMKYQL
   301  LLKDFLKYYN RAGMDTADLE QAVEVMCFVP KRCNDMMTLG RLRGFEGKLT AQGKLLGQDT
   361  FWVTEPEAGG LLSSRGRERR VFLFEQIIIF SEALGGGVRG GTQPGYVYKN SIKVSCLGLE
   421  GNLQGDPCRF ALTSRGPEGG IQRYVLQAAD PAISQAWIKH VAQILESQRD FLNALQSPIE
   481  YQRRESQTNS LGRPRGPGVG SPGRIQLGDQ AQGSTHTPIN GSLPSLLLSP KGEVARALLP
   541  LDKQALGDIP QAPHDSPPVS PTPKTPPCQA RLAKLDEDEL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGEF25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.45
Highest tissue expression: 144 nTPM

Expression across tissuesHPA

Tissue

colon: 144 nTPM
blood vessel: 115 nTPM
cerebellum: 113 nTPM
fallopian tube: 102 nTPM
endometrium: 96 nTPM
urinary bladder: 91 nTPM

Single-cell type

vascular smooth muscle cells: 7.8 nCPM
pericytes: 5.4 nCPM
smooth muscle cells: 5.1 nCPM
podocytes: 3.4 nCPM
endometrial stromal cells: 3.3 nCPM
fibro-adipogenic progenitors: 3.3 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

hippocampal formation: 137 nTPM
cerebral cortex: 135 nTPM
white matter: 68 nTPM
cerebellum: 65 nTPM
basal ganglia: 59 nTPM
amygdala: 50 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ARHGEF25.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 104 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Moyamoya angiopathy

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.85
gnomAD pLI: 0
gnomAD missense Z: 0.93
DepMap mean gene effect: -0.1
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

guanyl-nucleotide exchange factor activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARHGEF25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARHGEF25 as an antibody target. Whether an autoantibody or antibody against ARHGEF25 could matter depends on whether native ARHGEF25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARHGEF25 is annotated at the cell surface, where native ARHGEF25 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ARHGEF25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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