Seroatlas · Human Serome Atlas

CNKSR1

Connector enhancer of kinase suppressor of ras 1

Also known as: CNK, CNK1, CNKR1_HUMAN, KSR

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q969H4
Gene: CNKSR1
Ensembl: ENSG00000142675
Chromosome: 1
Canonical length: 720 aa
Protein class: Cancer-related genes, Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

This gene encodes a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Canonical amino-acid sequenceUniProt

720 residues, UniProt reviewed canonical sequence.

>Q969H4|CNKSR1
     1  MEPVETWTPG KVATWLRGLD DSLQDYPFED WQLPGKNLLQ LCPQSLEALA VRSLGHQELI
    61  LGGVEQLQAL SSRLQTENLQ SLTEGLLGAT HDFQSIVQGC LGDCAKTPID VLCAAVELLH
   121  EADALLFWLS RYLFSHLNDF SACQEIRDLL EELSQVLHED GPAAEKEGTV LRICSHVAGI
   181  CHNILVCCPK ELLEQKAVLE QVQLDSPLGL EIHTTSNCQH FVSQVDTQVP TDSRLQIQPG
   241  DEVVQINEQV VVREERDMVG WPRKNMVREL LREPAGLSLV LKKIPIPETP PQTPPQVLDS
   301  PHQRSPSLSL APLSPRAPSE DVFAFDLSSN PSPGPSPAWT DSASLGPEPL PIPPEPPAIL
   361  PAGVAGTPGL PESPDKSPVG RKKSKGLATR LSRRRVSCRE LGRPDCDGWL LLRKAPGGFM
   421  GPRWRRRWFV LKGHTLYWYR QPQDEKAEGL INVSNYSLES GHDQKKKYVF QLTHDVYKPF
   481  IFAADTLTDL SMWVRHLITC ISKYQSPGRA PPPREEDCYS ETEAEDPDDE AGSHSASPSP
   541  AQAGSPLHGD TSPAATPTQR SPRTSFGSLT DSSEEALEGM VRGLRQGGVS LLGQPQPLTQ
   601  EQWRSSFMRR NRDPQLNERV HRVRALQSTL KAKLQELQVL EEVLGDPELT GEKFRQWKEQ
   661  NRELYSEGLG AWGVAQAEGS SHILTSDSTE QSPHSLPSDP EEHSHLCPLT SESSLRPPDL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CNKSR1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.43
Highest tissue expression: 54 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 54 nTPM
esophagus: 22 nTPM
pancreas: 19 nTPM
thyroid gland: 19 nTPM
salivary gland: 18 nTPM
skin: 16 nTPM

Single-cell type

esophageal apical cells: 106 nCPM
alveolar cells type 1: 67 nCPM
enterocytes: 62 nCPM
ocular epithelial cells: 60 nCPM
cytotrophoblasts: 56 nCPM
respiratory ciliated cells: 53 nCPM

Immune cell

T-reg: 0.8 nTPM
memory CD4 T-cell: 0.6 nTPM
memory B-cell: 0.5 nTPM
naive CD4 T-cell: 0.3 nTPM
NK-cell: 0.1 nTPM
total PBMC: 0.1 nTPM

Brain region

cerebellum: 6.9 nTPM
white matter: 5.7 nTPM
cerebral cortex: 4.6 nTPM
medulla oblongata: 4.3 nTPM
basal ganglia: 4.2 nTPM
pons: 3.8 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CNKSR1.

Disease | GeneticClinVar

2 pathogenic / likely-pathogenic of 132 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

CNKSR1-related disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.28
gnomAD pLI: 0
gnomAD missense Z: 0.57
DepMap mean gene effect: -0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

cell surface receptor protein tyrosine kinase signaling pathway

Molecular functions

protein-macromolecule adaptor activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CNKSR1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CNKSR1 as an antibody target. Whether an autoantibody or antibody against CNKSR1 could matter depends on whether native CNKSR1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CNKSR1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CNKSR1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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