ARHGDIB

Protein identityUniProt · HPA

UniProt accession

P52566

Gene

ARHGDIB

Ensembl

ENSG00000111348

Chromosome

12

Canonical length

201 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Cytosol

OverviewNCBI Gene

Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).[supplied by OMIM, Dec 2010]

Canonical amino-acid sequenceUniProt

201 residues, UniProt reviewed canonical sequence.

>P52566|ARHGDIB
     1  MTEKAPEPHV EEDDDDELDS KLNYKPPPQK SLKELQEMDK DDESLIKYKK TLLGDGPVVT
    61  DPKAPNVVVT RLTLVCESAP GPITMDLTGD LEALKKETIV LKEGSEYRVK IHFKVNRDIV
   121  SGLKYVQHTY RTGVKVDKAT FMVGSYGPRP EEYEFLTPVE EAPKGMLARG TYHNKSFFTD
   181  DDKQDHLSWE WNLSIKKEWT E

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGDIB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.34

Highest tissue expression

1,491 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for ARHGDIB from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.3

gnomAD pLI

0

gnomAD missense Z

0.04

DepMap mean gene effect

0.04

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to redox state
• negative regulation of trophoblast cell migration
• regulation of Rho protein signal transduction
• Rho protein signal transduction

Molecular functions

• GTPase activator activity
• GTPase activity
• Rho GDP-dissociation inhibitor activity
• small GTPase binding

Cellular components

• cytoplasm
• cytoplasmic vesicle
• cytoskeleton
• cytosol
• extracellular exosome
• membrane

Protein domainsUniProt · Pfam · InterPro

• Rho protein GDP-dissociation inhibitor
• Immunoglobulin E-set
• Rho GDP-dissociation inhibitor domain superfamily
• RHO protein GDP dissociation inhibitor

KeywordsUniProt

• Acetylation
• Cytoplasm
• GTPase activation
• Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of ARHGDIB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CDC42
• RAC1
• RAC2
• RHOA