Seroatlas · Human Serome Atlas

ARHGEF4

Rho guanine nucleotide exchange factor 4

Also known as: ARHG4_HUMAN, ASEF, ASEF1, KIAA1112, STM6

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NR80
Gene
ARHGEF4
Ensembl
ENSG00000136002
Chromosome
2
Canonical length
690 aa
Protein class
Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013]

Canonical amino-acid sequenceUniProt

690 residues, UniProt reviewed canonical sequence.

>Q9NR80|ARHGEF4
     1  MPWEEPAGEK PSCSHSQKAF HMEPAQKPCF TTDMVTWALL CISAETVRGE APSQPRGIPH
    61  RSPVSVDDLW LEKTQRKKLQ KQAHVERRLH IGAVHKDGVK CWRKTIITSP ESLNLPRRSH
   121  PLSQSAPTGL NHMGWPEHTP GTAMPDGALD TAVCADEVGS EEDLYDDLHS SSHHYSHPGG
   181  GGEQLAINEL ISDGSVVCAE ALWDHVTMDD QELGFKAGDV IEVMDATNRE WWWGRVADGE
   241  GWFPASFVRL RVNQDEPADD DAPLAGNSGA EDGGAEAQSS KDQMRTNVIN EILSTERDYI
   301  KHLRDICEGY VRQCRKRADM FSEEQLRTIF GNIEDIYRCQ KAFVKALEQR FNRERPHLSE
   361  LGACFLEHQA DFQIYSEYCN NHPNACVELS RLTKLSKYVY FFEACRLLQK MIDISLDGFL
   421  LTPVQKICKY PLQLAELLKY THPQHRDFKD VEAALHAMKN VAQLINERKR RLENIDKIAQ
   481  WQSSIEDWEG EDLLVRSSEL IYSGELTRVT QPQAKSQQRM FFLFDHQLIY CKKDLLRRDV
   541  LYYKGRLDMD GLEVVDLEDG KDRDLHVSIK NAFRLHRGAT GDSHLLCTRK PEQKQRWLKA
   601  FAREREQVQL DQETGFSITE LQRKQAMLNA SKQQVTGKPK AVGRPCYLTR QKHPALPSNR
   661  PQQQVLVLAE PRRKPSTFWH SISRLAPFRK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGEF4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.43
Highest tissue expression
96 nTPM

Expression across tissuesHPA

Tissue

  • hippocampal formation: 96 nTPM
  • cerebral cortex: 93 nTPM
  • amygdala: 80 nTPM
  • basal ganglia: 80 nTPM
  • hypothalamus: 41 nTPM
  • skin: 38 nTPM

Single-cell type

  • astrocytes: 541 nCPM
  • choroid plexus epithelial cells: 291 nCPM
  • oligodendrocyte progenitor cells: 257 nCPM
  • esophageal apical cells: 248 nCPM
  • suprabasal keratinocytes: 237 nCPM
  • ocular epithelial cells: 229 nCPM

Immune cell

  • naive CD8 T-cell: 1.4 nTPM
  • plasmacytoid DC: 1 nTPM
  • MAIT T-cell: 0.5 nTPM
  • memory CD8 T-cell: 0.5 nTPM
  • naive CD4 T-cell: 0.5 nTPM
  • T-reg: 0.5 nTPM

Brain region

  • cerebral cortex: 118 nTPM
  • hippocampal formation: 116 nTPM
  • basal ganglia: 102 nTPM
  • amygdala: 94 nTPM
  • thalamus: 78 nTPM
  • white matter: 78 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.55
gnomAD pLI
0
gnomAD missense Z
1.33
DepMap mean gene effect
0
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARHGEF4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARHGEF4 as an antibody target. Whether an autoantibody or antibody against ARHGEF4 could matter depends on whether native ARHGEF4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARHGEF4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARHGEF4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ARHGEF4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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