Seroatlas · Human Serome Atlas

ARHGEF5

Rho guanine nucleotide exchange factor 5

Also known as: ARHG5_HUMAN, GEF5, P60, TIM, TIM1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q12774
Gene: ARHGEF5
Ensembl: ENSG00000050327
Chromosome: 7
Canonical length: 1597 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Plasma membrane,Cytosol

OverviewNCBI Gene

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1597 residues, UniProt reviewed canonical sequence.

>Q12774|ARHGEF5
     1  MEAEEAQRGA SPPISAIEEF SIIPEAPMRS SQVSALGLEA QEDEDPSYKW REEHRLSATQ
    61  QSELRDVCDY AIETMPSFPK EGSADVEPNQ ESLVAEACDT PEHWEAVPQS LAGRQARTLA
   121  PPELWACPIQ SEHLDMAPFS SDLGSEEEEV EFWPGLTSLT LGSGQAEEEE ETSSDNSGQT
   181  RYYSPCEEHP AETNQNEGSE SGTIRQGEEL PPEELQESQG LLHPQEVQVL EEQGQQEAGF
   241  RGEGTLREDV CADGLLGEEQ MIEQVNDEKG EQKQKQEQVQ DVMLGRQGER MGLTGEPEGL
   301  NDGEWEQEDM ERKAQGQGGP EQGEERKREL QVPEENRADS QDEKSQTFLG KSEEVTGKQE
   361  DHGIKEKGVP VSGQEAKEPE SWDGGRLGAV GRARSREEEN EHHGPSMPAL IAPEDSPHCD
   421  LFPGASYLMT QIPGTQTESR AEELSPAALS PSLEPIRCSH QPISLLGSFL TEESPDKEID
   481  QNSQQEESRL RKGTVSSQGT EVVFASASVT PPRTPDSAPP SPAEAYPITP ASVSARPPVA
   541  FPRRETSCAA RAPETASAPL SMDDPSPCGT SEMCPAALYG FPSTGTSPPR PPANSTGTVQ
   601  HLRSDSFPGS HRTEQTPDLV GMLLSYSHSE LPQRPPKPAI YSSVTPRRDR RSGRDYSTVS
   661  ASPTALSTLK QDSQESISNL ERPSSPPSIQ PWVSPHNPAF ATESPAYGSS PSFVSMEDVR
   721  IHEPLPPPPP QRRDTHPSVV ETDGHARVVV PTLKQHSHPP PLALGSGLHA PHKGPLPQAS
   781  DPAVARQHRP LPSTPDSSHH AQATPRWRYN KPLPPTPDLP QPHLPPISAP GSSRIYRPLP
   841  PLPIIDPPTE PPPLPPKSRG RSRSTRGGHM NSGGHAKTRP ACQDWTVPLP ASAGRTSWPP
   901  ATARSTESFT STSRSKSEVS PGMAFSNMTN FLCPSSPTTP WTPELQGPTS KDEAGVSEHP
   961  EAPAREPLRR TTPQQGASGP GRSPVGQARQ PEKPSHLHLE KASSWPHRRD SGRPPGDSSG
  1021  QAVAPSEGAN KHKGWSRQGL RRPSILPEGS SDSRGPAVEK HPGPSDTVVF REKKPKEVMG
  1081  GFSRRCSKLI NSSQLLYQEY SDVVLNKEIQ SQQRLESLSE TPGPSSPRQP RKALVSSESY
  1141  LQRLSMASSG SLWQEIPVVR NSTVLLSMTH EDQKLQEVKF ELIVSEASYL RSLNIAVDHF
  1201  QLSTSLRATL SNQEHQWLFS RLQDVRDVSA TFLSDLEENF ENNIFSFQVC DVVLNHAPDF
  1261  RRVYLPYVTN QTYQERTFQS LMNSNSNFRE VLEKLESDPV CQRLSLKSFL ILPFQRITRL
  1321  KLLLQNILKR TQPGSSEEAE ATKAHHALEQ LIRDCNNNVQ SMRRTEELIY LSQKIEFECK
  1381  IFPLISQSRW LVKSGELTAL EFSASPGLRR KLNTRPVHLH LFNDCLLLSR PREGSRFLVF
  1441  DHAPFSSIRG EKCEMKLHGP HKNLFRLFLR QNTQGAQAEF LFRTETQSEK LRWISALAMP
  1501  REELDLLECY NSPQVQCLRA YKPRENDELA LEKADVVMVT QQSSDGWLEG VRLSDGERGW
  1561  FPVQQVEFIS NPEVRAQNLK EAHRVKTAKL QLVEQQA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGEF5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.57
Highest tissue expression: 45 nTPM

Expression across tissuesHPA

Tissue

skin: 45 nTPM
esophagus: 36 nTPM
thyroid gland: 23 nTPM
kidney: 21 nTPM
pancreas: 18 nTPM
salivary gland: 18 nTPM

Single-cell type

loop of henle epithelial cells: 16 nCPM
renal collecting duct principal cells: 15 nCPM
ocular epithelial cells: 14 nCPM
renal connecting tubule cells: 13 nCPM
proximal tubule cells: 13 nCPM
renal collecting duct intercalated cells: 13 nCPM

Immune cell

T-reg: 1.8 nTPM
naive B-cell: 0.9 nTPM
naive CD4 T-cell: 0.9 nTPM
memory B-cell: 0.8 nTPM
memory CD4 T-cell: 0.8 nTPM
memory CD8 T-cell: 0.7 nTPM

Brain region

choroid plexus: 13 nTPM
midbrain: 6.4 nTPM
basal ganglia: 5.4 nTPM
medulla oblongata: 5.4 nTPM
thalamus: 4.7 nTPM
pons: 4.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.98
gnomAD pLI: 0
gnomAD missense Z: 1.47
DepMap mean gene effect: -0.38
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARHGEF5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARHGEF5 as an antibody target. Whether an autoantibody or antibody against ARHGEF5 could matter depends on whether native ARHGEF5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARHGEF5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARHGEF5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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