Seroatlas · Human Serome Atlas

PSMD3

26S proteasome non-ATPase regulatory subunit 3

Also known as: P58, PSMD3_HUMAN, Rpn3, S3, TSTA2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O43242
Gene: PSMD3
Ensembl: ENSG00000108344
Chromosome: 17
Canonical length: 534 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in this gene are associated with neutrophil count. [provided by RefSeq, Jul 2012]

Canonical amino-acid sequenceUniProt

534 residues, UniProt reviewed canonical sequence.

>O43242|PSMD3
     1  MKQEGSARRR GADKAKPPPG GGEQEPPPPP APQDVEMKEE AATGGGSTGE ADGKTAAAAA
    61  EHSQRELDTV TLEDIKEHVK QLEKAVSGKE PRFVLRALRM LPSTSRRLNH YVLYKAVQGF
   121  FTSNNATRDF LLPFLEEPMD TEADLQFRPR TGKAASTPLL PEVEAYLQLL VVIFMMNSKR
   181  YKEAQKISDD LMQKISTQNR RALDLVAAKC YYYHARVYEF LDKLDVVRSF LHARLRTATL
   241  RHDADGQATL LNLLLRNYLH YSLYDQAEKL VSKSVFPEQA NNNEWARYLY YTGRIKAIQL
   301  EYSEARRTMT NALRKAPQHT AVGFKQTVHK LLIVVELLLG EIPDRLQFRQ PSLKRSLMPY
   361  FLLTQAVRTG NLAKFNQVLD QFGEKFQADG TYTLIIRLRH NVIKTGVRMI SLSYSRISLA
   421  DIAQKLQLDS PEDAEFIVAK AIRDGVIEAS INHEKGYVQS KEMIDIYSTR EPQLAFHQRI
   481  SFCLDIHNMS VKAMRFPPKS YNKDLESAEE RREREQQDLE FAKEMAEDDD DSFP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PSMD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 242 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 242 nTPM
tongue: 97 nTPM
liver: 89 nTPM
skin: 82 nTPM
heart muscle: 73 nTPM
esophagus: 67 nTPM

Single-cell type

syncytiotrophoblasts: 331 nCPM
early spermatids: 286 nCPM
cytotrophoblasts: 196 nCPM
migrating cytotrophoblasts: 171 nCPM
extravillous trophoblasts: 150 nCPM
late primary spermatocytes: 139 nCPM

Immune cell

myeloid DC: 30 nTPM
classical monocyte: 23 nTPM
intermediate monocyte: 21 nTPM
memory CD8 T-cell: 20 nTPM
plasmacytoid DC: 20 nTPM
naive CD8 T-cell: 19 nTPM

Brain region

cerebral cortex: 74 nTPM
pons: 73 nTPM
hypothalamus: 70 nTPM
white matter: 67 nTPM
medulla oblongata: 66 nTPM
hippocampal formation: 66 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.1
gnomAD pLI: 1
gnomAD missense Z: 2.28
DepMap mean gene effect: -1.71
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

enzyme regulator activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

Proteasome component (PCI) domain
Tetratricopeptide-like helical domain superfamily
Winged helix DNA-binding domain superfamily
COP9 signalosome complex subunit 3
PCI domain
26S proteasome non-ATPase regulatory subunit 3, C-terminal
26S proteasome non-ATPase regulatory subunit 3, N-terminal TPR repeats
Proteasome regulatory subunit C-terminal
PSMD3-like, N-terminal TPR repeats

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PSMD3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PSMD3 as an antibody target. Whether an autoantibody or antibody against PSMD3 could matter depends on whether native PSMD3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PSMD3 is annotated as secreted, so native PSMD3 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label PSMD3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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