Seroatlas · Human Serome Atlas

PAAF1

Proteasomal ATPase-associated factor 1

Also known as: FLJ11848, PAAF1_HUMAN, Rpn14, WDR71

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9BRP4
Gene: PAAF1
Ensembl: ENSG00000175575
Chromosome: 11
Canonical length: 392 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a WD repeat-containing protein involved in regulation of association of proteasome components. During HIV infection, the encoded protein is thought to promote provirus transcription through recruitment of the 19S regulatory complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012]

Canonical amino-acid sequenceUniProt

392 residues, UniProt reviewed canonical sequence.

>Q9BRP4|PAAF1
     1  MAAPLRIQSD WAQALRKDEG EAWLSCHPPG KPSLYGSLTC QGIGLDGIPE VTASEGFTVN
    61  EINKKSIHIS CPKENASSKF LAPYTTFSRI HTKSITCLDI SSRGGLGVSS STDGTMKIWQ
   121  ASNGELRRVL EGHVFDVNCC RFFPSGLVVL SGGMDAQLKI WSAEDASCVV TFKGHKGGIL
   181  DTAIVDRGRN VVSASRDGTA RLWDCGRSAC LGVLADCGSS INGVAVGAAD NSINLGSPEQ
   241  MPSEREVGTE AKMLLLARED KKLQCLGLQS RQLVFLFIGS DAFNCCTFLS GFLLLAGTQD
   301  GNIYQLDVRS PRAPVQVIHR SGAPVLSLLS VRDGFIASQG DGSCFIVQQD LDYVTELTGA
   361  DCDPVYKVAT WEKQIYTCCR DGLVRRYQLS DL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PAAF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.22
Highest tissue expression: 111 nTPM

Expression across tissuesHPA

Tissue

spinal cord: 111 nTPM
midbrain: 100 nTPM
amygdala: 80 nTPM
basal ganglia: 76 nTPM
hippocampal formation: 75 nTPM
hypothalamus: 72 nTPM

Single-cell type

myonuclei: 126 nCPM
oligodendrocytes: 80 nCPM
sertoli cells: 72 nCPM
bergmann glia: 70 nCPM
astrocytes: 67 nCPM
early spermatids: 65 nCPM

Immune cell

basophil: 53 nTPM
memory B-cell: 41 nTPM
naive CD4 T-cell: 37 nTPM
T-reg: 37 nTPM
naive B-cell: 36 nTPM
myeloid DC: 35 nTPM

Brain region

white matter: 91 nTPM
basal ganglia: 75 nTPM
midbrain: 66 nTPM
medulla oblongata: 65 nTPM
pons: 63 nTPM
spinal cord: 61 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.21
gnomAD pLI: 0
gnomAD missense Z: 0.93
DepMap mean gene effect: -0.12
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PAAF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PAAF1 as an antibody target. Whether an autoantibody or antibody against PAAF1 could matter depends on whether native PAAF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PAAF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PAAF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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