Seroatlas · Human Serome Atlas

RPL27

Large ribosomal subunit protein eL27

Also known as: L27, RL27_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P61353
Gene
RPL27
Ensembl
ENSG00000131469
Chromosome
17
Canonical length
136 aa
Protein class
Cancer-related genes, Disease related genes, Human disease related genes, Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Nucleoplasm,Nucleoli

OverviewNCBI Gene

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Mar 2017]

Canonical amino-acid sequenceUniProt

136 residues, UniProt reviewed canonical sequence.

>P61353|RPL27
     1  MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK
    61  KIAKRSKIKS FVKVYNYNHL MPTRYSVDIP LDKTVVNKDV FRDPALKRKA RREAKVKFEE
   121  RYKTGKNKWF FQKLRF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPL27 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.32
Highest tissue expression
2,759 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 2,759 nTPM
  • ovary: 2,512 nTPM
  • skin: 2,484 nTPM
  • skeletal muscle: 2,362 nTPM
  • pancreas: 2,050 nTPM
  • lymph node: 1,868 nTPM

Single-cell type

  • esophageal suprabasal cells: 5,693 nCPM
  • esophageal basal cells: 4,679 nCPM
  • extravillous trophoblasts: 4,444 nCPM
  • esophageal apical cells: 3,819 nCPM
  • decidual stromal cells: 3,488 nCPM
  • migrating cytotrophoblasts: 3,217 nCPM

Immune cell

  • total PBMC: 4,611 nTPM
  • memory B-cell: 3,067 nTPM
  • naive CD4 T-cell: 2,997 nTPM
  • naive B-cell: 2,804 nTPM
  • plasmacytoid DC: 2,734 nTPM
  • naive CD8 T-cell: 2,683 nTPM

Brain region

  • white matter: 304 nTPM
  • thalamus: 300 nTPM
  • hypothalamus: 296 nTPM
  • spinal cord: 293 nTPM
  • medulla oblongata: 291 nTPM
  • pons: 274 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RPL27.

Disease | AllUniProt

Conditions RPL27 is implicated in, by any mechanism.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 86 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Diamond-Blackfan anemia 16

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.48
gnomAD pLI
0.85
gnomAD missense Z
1.14
DepMap mean gene effect
-2.05
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPL27 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPL27 as an antibody target. Whether an autoantibody or antibody against RPL27 could matter depends on whether native RPL27 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPL27 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPL27 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPL27. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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