Seroatlas · Human Serome Atlas

RPL32

Large ribosomal subunit protein eL32

Also known as: L32, RL32_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P62910
Gene
RPL32
Ensembl
ENSG00000144713
Chromosome
3
Canonical length
135 aa
Protein class
Plasma proteins, Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

135 residues, UniProt reviewed canonical sequence.

>P62910|RPL32
     1  MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY
    61  GSNKKTKHML PSGFRKFLVH NVKELEVLLM CNKSYCAEIA HNVSSKNRKA IVERAAQLAI
   121  RVTNPNARLR SEENE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPL32 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
3,327 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 3,327 nTPM
  • bone marrow: 2,802 nTPM
  • tonsil: 2,791 nTPM
  • pancreas: 2,624 nTPM
  • skin: 2,501 nTPM
  • breast: 2,353 nTPM

Single-cell type

  • esophageal suprabasal cells: 12,675 nCPM
  • late spermatids: 11,651 nCPM
  • decidual stromal cells: 11,510 nCPM
  • esophageal basal cells: 11,302 nCPM
  • gastric progenitor cells: 10,898 nCPM
  • extravillous trophoblasts: 9,289 nCPM

Immune cell

  • total PBMC: 9,498 nTPM
  • naive CD4 T-cell: 6,684 nTPM
  • memory B-cell: 6,007 nTPM
  • naive B-cell: 5,439 nTPM
  • naive CD8 T-cell: 5,235 nTPM
  • memory CD4 T-cell: 4,766 nTPM

Brain region

  • white matter: 647 nTPM
  • spinal cord: 621 nTPM
  • medulla oblongata: 582 nTPM
  • basal ganglia: 579 nTPM
  • thalamus: 541 nTPM
  • hypothalamus: 539 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.56
gnomAD pLI
0.8
gnomAD missense Z
0.55
DepMap mean gene effect
-2.27
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Large ribosomal subunit protein eL32
  • Large ribosomal subunit protein eL32, conserved site
  • Large ribosomal subunit protein eL32 superfamily
  • Ribosomal protein L32

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPL32 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPL32 as an antibody target. Whether an autoantibody or antibody against RPL32 could matter depends on whether native RPL32 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPL32 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPL32 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPL32. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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