RAB8A
Ras-related protein Rab-8A
Also known as: MEL, RAB8, RAB8A_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P61006
- Gene
- RAB8A
- Ensembl
- ENSG00000167461
- Chromosome
- 19
- Canonical length
- 207 aa
- Protein class
- Enzymes, Predicted intracellular proteins
- Subcellular location
- Nucleoplasm,Nucleoli,Golgi apparatus,Plasma membrane,Actin filaments,Primary cilium,Centriolar satellite,Basal body,Cytosol
OverviewNCBI Gene
The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
207 residues, UniProt reviewed canonical sequence.
>P61006|RAB8A
1 MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI ELDGKRIKLQ
61 IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR NWIRNIEEHA SADVEKMILG
121 NKCDVNDKRQ VSKERGEKLA LDYGIKFMET SAKANINVEN AFFTLARDIK AKMDKKLEGN
181 SPQGSNQGVK ITPDQQKRSS FFRCVLLLocalizationUniProt · AlphaFold · HPA
Whether an antibody against RAB8A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Cell surface
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.33
- Highest tissue expression
- 58 nTPM
Expression across tissuesHPA
Tissue
- bone marrow: 58 nTPM
- small intestine: 55 nTPM
- duodenum: 55 nTPM
- liver: 48 nTPM
- tonsil: 42 nTPM
- lymph node: 41 nTPM
Single-cell type
- platelets: 661 nCPM
- megakaryocytes: 365 nCPM
- megakaryocyte progenitors: 190 nCPM
- plasma cells: 135 nCPM
- enterocytes: 130 nCPM
- extravillous trophoblasts: 120 nCPM
Immune cell
- total PBMC: 514 nTPM
- non-classical monocyte: 488 nTPM
- intermediate monocyte: 388 nTPM
- eosinophil: 316 nTPM
- neutrophil: 293 nTPM
- classical monocyte: 279 nTPM
Brain region
- white matter: 37 nTPM
- medulla oblongata: 33 nTPM
- choroid plexus: 31 nTPM
- spinal cord: 30 nTPM
- pons: 30 nTPM
- midbrain: 29 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.26
- gnomAD pLI
- 0.98
- gnomAD missense Z
- 2.12
- DepMap mean gene effect
- -0.18
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- autophagy
- axonogenesis
- cellular response to insulin stimulus
- cilium assembly
- endocytic recycling
- exocytosis
- Golgi organization
- Golgi vesicle fusion to target membrane
- neurotransmitter receptor transport to postsynaptic membrane
- neurotransmitter receptor transport, endosome to postsynaptic membrane
- protein localization to cilium
- protein localization to plasma membrane
- regulation of autophagy
- regulation of long-term neuronal synaptic plasticity
- regulation of protein transport
- vesicle docking involved in exocytosis
- vesicle-mediated transport in synapse
Molecular functions
- G protein activity
- GDP binding
- GTP binding
- GTPase activity
- myosin V binding
- protein tyrosine kinase binding
- small GTPase binding
Cellular components
- actin cytoskeleton
- axoneme
- centriolar satellite
- centriole
- centrosome
- ciliary basal body
- ciliary base
- ciliary membrane
- cilium
- cytosol
- dendrite
- endosome
- endosome membrane
- extracellular exosome
- glutamatergic synapse
- Golgi apparatus
- Golgi membrane
- lysosome
- midbody
- neuronal cell body
- non-motile cilium
- nucleolus
- nucleoplasm
- phagocytic vesicle
- phagocytic vesicle membrane
- plasma membrane
- recycling endosome membrane
- synaptic vesicle
- trans-Golgi network membrane
- trans-Golgi network transport vesicle
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of RAB8A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads RAB8A as an antibody target. Whether an autoantibody or antibody against RAB8A could matter depends on whether native RAB8A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
RAB8A is annotated at the cell surface, where native RAB8A is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.
Annotation status
The present source text does not explicitly label RAB8A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
Loading the interactive Seroatlas protein explorer...