Seroatlas · Human Serome Atlas

MAP4K2

Mitogen-activated protein kinase kinase kinase kinase 2

Also known as: BL44, GCK, M4K2_HUMAN, RAB8IP

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q12851
Gene
MAP4K2
Ensembl
ENSG00000168067
Chromosome
11
Canonical length
820 aa
Protein class
Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Vesicles

OverviewNCBI Gene

The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Canonical amino-acid sequenceUniProt

820 residues, UniProt reviewed canonical sequence.

>Q12851|MAP4K2
     1  MALLRDVSLQ DPRDRFELLQ RVGAGTYGDV YKARDTVTSE LAAVKIVKLD PGDDISSLQQ
    61  EITILRECRH PNVVAYIGSY LRNDRLWICM EFCGGGSLQE IYHATGPLEE RQIAYVCREA
   121  LKGLHHLHSQ GKIHRDIKGA NLLLTLQGDV KLADFGVSGE LTASVAKRRS FIGTPYWMAP
   181  EVAAVERKGG YNELCDVWAL GITAIELGEL QPPLFHLHPM RALMLMSKSS FQPPKLRDKT
   241  RWTQNFHHFL KLALTKNPKK RPTAEKLLQH PFTTQQLPRA LLTQLLDKAS DPHLGTPSPE
   301  DCELETYDMF PDTIHSRGQH GPAERTPSEI QFHQVKFGAP RRKETDPLNE PWEEEWTLLG
   361  KEELSGSLLQ SVQEALEERS LTIRSASEFQ ELDSPDDTMG TIKRAPFLGP LPTDPPAEEP
   421  LSSPPGTLPP PPSGPNSSPL LPTAWATMKQ REDPERSSCH GLPPTPKVHM GACFSKVFNG
   481  CPLRIHAAVT WIHPVTRDQF LVVGAEEGIY TLNLHELHED TLEKLISHRC SWLYCVNNVL
   541  LSLSGKSTHI WAHDLPGLFE QRRLQQQVPL SIPTNRLTQR IIPRRFALST KIPDTKGCLQ
   601  CRVVRNPYTG ATFLLAALPT SLLLLQWYEP LQKFLLLKNF SSPLPSPAGM LEPLVLDGKE
   661  LPQVCVGAEG PEGPGCRVLF HVLPLEAGLT PDILIPPEGI PGSAQQVIQV DRDTILVSFE
   721  RCVRIVNMQG EPTATLAPEL TFDFPIETVV CLQDSVLAFW SHGMQGRSLD TNEVTQEITD
   781  ETRIFRVLGA HRDIILESIP TDNPEAHSNL YILTGHQSTY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAP4K2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.31
Highest tissue expression
18 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 18 nTPM
  • lymph node: 17 nTPM
  • spleen: 16 nTPM
  • bone marrow: 15 nTPM
  • tonsil: 13 nTPM
  • cerebral cortex: 11 nTPM

Single-cell type

  • platelets: 86 nCPM
  • lymphatic endothelial cells: 75 nCPM
  • late spermatids: 55 nCPM
  • hematopoietic stem cells: 45 nCPM
  • early spermatids: 43 nCPM
  • b-cells: 40 nCPM

Immune cell

  • non-classical monocyte: 16 nTPM
  • intermediate monocyte: 13 nTPM
  • memory B-cell: 9.9 nTPM
  • T-reg: 9.5 nTPM
  • neutrophil: 8 nTPM
  • naive CD4 T-cell: 7.7 nTPM

Brain region

  • cerebral cortex: 21 nTPM
  • thalamus: 20 nTPM
  • cerebellum: 20 nTPM
  • amygdala: 19 nTPM
  • white matter: 18 nTPM
  • midbrain: 17 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.69
gnomAD pLI
0
gnomAD missense Z
1.6
DepMap mean gene effect
-0.17
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MAP4K2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MAP4K2 as an antibody target. Whether an autoantibody or antibody against MAP4K2 could matter depends on whether native MAP4K2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MAP4K2 is annotated at the cell surface, where native MAP4K2 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label MAP4K2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MAP4K2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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