RPH3A

Protein identityUniProt · HPA

UniProt accession

Q9Y2J0

Gene

RPH3A

Ensembl

ENSG00000089169

Chromosome

12

Canonical length

694 aa

Protein class

Predicted intracellular proteins

OverviewNCBI Gene

The protein encoded by this gene is thought to be an effector for RAB3A, which is a small G protein that acts in the late stages of neurotransmitter exocytosis. The encoded protein may be involved in neurotransmitter release and synaptic vesicle traffic. [provided by RefSeq, Dec 2016]

Canonical amino-acid sequenceUniProt

694 residues, UniProt reviewed canonical sequence.

>Q9Y2J0|RPH3A
     1  MTDTVFSNSS NRWMYPSDRP LQSNDKEQLQ AGWSVHPGGQ PDRQRKQEEL TDEEKEIINR
    61  VIARAEKMEE MEQERIGRLV DRLENMRKNV AGDGVNRCIL CGEQLGMLGS ACVVCEDCKK
   121  NVCTKCGVET NNRLHSVWLC KICIEQREVW KRSGAWFFKG FPKQVLPQPM PIKKTKPQQP
   181  VSEPAAPEQP APEPKHPARA PARGDSEDRR GPGQKTGPDP ASAPGRGNYG PPVRRASEAR
   241  MSSSSRDSES WDHSGGAGDS SRSPAGLRRA NSVQASRPAP GSVQSPAPPQ PGQPGTPGGS
   301  RPGPGPAGRF PDQKPEVAPS DPGTTAPPRE ERTGGVGGYP AVGAREDRMS HPSGPYSQAS
   361  AAAPQPAAAR QPPPPEEEEE EANSYDSDEA TTLGALEFSL LYDQDNSSLQ CTIIKAKGLK
   421  PMDSNGLADP YVKLHLLPGA SKSNKLRTKT LRNTRNPIWN ETLVYHGITD EDMQRKTLRI
   481  SVCDEDKFGH NEFIGETRFS LKKLKPNQRK NFNICLERVI PMKRAGTTGS ARGMALYEEE
   541  QVERVGDIEE RGKILVSLMY STQQGGLIVG IIRCVHLAAM DANGYSDPFV KLWLKPDMGK
   601  KAKHKTQIKK KTLNPEFNEE FFYDIKHSDL AKKSLDISVW DYDIGKSNDY IGGCQLGISA
   661  KGERLKHWYE CLKNKDKKIE RWHQLQNENH VSSD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPH3A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.47

Highest tissue expression

115 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RPH3A.

Disease | AutoantibodyPubMed

Conditions in which antibodies against RPH3A are reported. Each links to that disease's full target list.

Showing 0 of 1 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for RPH3A from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.47

gnomAD pLI

0

gnomAD missense Z

1.31

DepMap mean gene effect

0.09

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• calcium-dependent activation of synaptic vesicle fusion
• intracellular protein transport
• positive regulation of calcium ion-dependent exocytosis
• spontaneous neurotransmitter secretion
• synaptic vesicle priming

Molecular functions

• calcium ion binding
• calcium-dependent phospholipid binding
• inositol 1,4,5 trisphosphate binding
• phosphate ion binding
• phosphatidylinositol phosphate binding
• phosphatidylinositol-4,5-bisphosphate binding
• protein-containing complex binding
• selenium binding
• small GTPase binding
• zinc ion binding

Cellular components

• dendritic spine
• extrinsic component of membrane
• extrinsic component of synaptic vesicle membrane
• neuron projection
• postsynaptic membrane
• protein-containing complex
• secretory granule
• synapse
• synaptic vesicle
• synaptic vesicle membrane

Protein domainsUniProt · Pfam · InterPro

• C2 domain
• Synaptotagmin
• Rab-binding domain
• Zinc finger, FYVE/PHD-type
• Zinc finger, RING/FYVE/PHD-type
• Zinc finger, FYVE-related
• C2 domain superfamily
• FYVE-type zinc finger
• Rabphilin/Doc2/Noc2
• Rabphilin/Doc2, first C2 domain
• C2 domain
• FYVE-type zinc finger
• Rabphilin-3A, FYVE domain

KeywordsUniProt

• Calcium
• Cell membrane
• Cell projection
• Cytoplasmic vesicle
• Lipid-binding
• Membrane
• Metal-binding
• Methylation
• Phosphoprotein
• Postsynaptic cell membrane
• Protein transport
• Repeat
• Synapse
• Transport
• Ubl conjugation
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of RPH3A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DLG4
• GRIN2A
• RAB27A
• RAB27B
• RAB3A
• RAB3B
• RAB3C
• RAB3D
• RAB8A
• SNCA
• CASK