Seroatlas · Human Serome Atlas

EHD1

EH domain-containing protein 1

Also known as: EHD1_HUMAN, FLJ42622, FLJ44618, H-PAST, HPAST1, PAST1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H4M9
Gene
EHD1
Ensembl
ENSG00000110047
Chromosome
11
Canonical length
534 aa
Protein class
Plasma proteins, Predicted intracellular proteins, Transporters
Subcellular location
Plasma membrane,Primary cilium,Primary cilium transition zone
Quaternary structure
Homooligomer

OverviewNCBI Gene

This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]

Canonical amino-acid sequenceUniProt

534 residues, UniProt reviewed canonical sequence.

>Q9H4M9|EHD1
     1  MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL EDADFDNKPM
    61  VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV MHGPTEGVVP GNALVVDPRR
   121  PFRKLNAFGN AFLNRFMCAQ LPNPVLDSIS IIDTPGILSG EKQRISRGYD FAAVLEWFAE
   181  RVDRIILLFD AHKLDISDEF SEVIKALKNH EDKIRVVLNK ADQIETQQLM RVYGALMWSL
   241  GKIINTPEVV RVYIGSFWSH PLLIPDNRKL FEAEEQDLFK DIQSLPRNAA LRKLNDLIKR
   301  ARLAKVHAYI ISSLKKEMPN VFGKESKKKE LVNNLGEIYQ KIEREHQISP GDFPSLRKMQ
   361  ELLQTQDFSK FQALKPKLLD TVDDMLANDI ARLMVMVRQE ESLMPSQVVK GGAFDGTMNG
   421  PFGHGYGEGA GEGIDDVEWV VGKDKPTYDE IFYTLSPVNG KITGANAKKE MVKSKLPNTV
   481  LGKIWKLADV DKDGLLDDEE FALANHLIKV KLEGHELPAD LPPHLVPPSK RRHE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EHD1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.27
Highest tissue expression
148 nTPM

Expression across tissuesHPA

Tissue

  • testis: 148 nTPM
  • bone marrow: 143 nTPM
  • spleen: 71 nTPM
  • lymph node: 61 nTPM
  • cerebellum: 58 nTPM
  • appendix: 56 nTPM

Single-cell type

  • late spermatids: 3,099 nCPM
  • early spermatids: 1,212 nCPM
  • neutrophils: 1,043 nCPM
  • monocytes: 338 nCPM
  • platelets: 325 nCPM
  • late primary spermatocytes: 289 nCPM

Immune cell

  • eosinophil: 100 nTPM
  • neutrophil: 57 nTPM
  • MAIT T-cell: 39 nTPM
  • gdT-cell: 39 nTPM
  • non-classical monocyte: 34 nTPM
  • memory CD8 T-cell: 34 nTPM

Brain region

  • cerebellum: 69 nTPM
  • cerebral cortex: 58 nTPM
  • medulla oblongata: 57 nTPM
  • white matter: 57 nTPM
  • spinal cord: 54 nTPM
  • thalamus: 53 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about EHD1.

Disease | ImmuneIEDB

Conditions an epitope on EHD1 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.28
gnomAD pLI
0.98
gnomAD missense Z
2.1
DepMap mean gene effect
-0.16
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EHD1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EHD1 as an antibody target. Whether an autoantibody or antibody against EHD1 could matter depends on whether native EHD1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EHD1 is annotated at the cell surface, where native EHD1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label EHD1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EHD1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...