Seroatlas · Human Serome Atlas

EIF2AK2

Interferon-induced, double-stranded RNA-activated protein kinase

Also known as: E2AK2_HUMAN, PKR, PPP1R83, PRKR

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P19525
Gene
EIF2AK2
Ensembl
ENSG00000055332
Chromosome
2
Canonical length
551 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins
Subcellular location
Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses. [provided by RefSeq, Jul 2021]

Canonical amino-acid sequenceUniProt

551 residues, UniProt reviewed canonical sequence.

>P19525|EIF2AK2
     1  MAGDLSAGFF MEELNTYRQK QGVVLKYQEL PNSGPPHDRR FTFQVIIDGR EFPEGEGRSK
    61  KEAKNAAAKL AVEILNKEKK AVSPLLLTTT NSSEGLSMGN YIGLINRIAQ KKRLTVNYEQ
   121  CASGVHGPEG FHYKCKMGQK EYSIGTGSTK QEAKQLAAKL AYLQILSEET SVKSDYLSSG
   181  SFATTCESQS NSLVTSTLAS ESSSEGDFSA DTSEINSNSD SLNSSSLLMN GLRNNQRKAK
   241  RSLAPRFDLP DMKETKYTVD KRFGMDFKEI ELIGSGGFGQ VFKAKHRIDG KTYVIKRVKY
   301  NNEKAEREVK ALAKLDHVNI VHYNGCWDGF DYDPETSDDS LESSDYDPEN SKNSSRSKTK
   361  CLFIQMEFCD KGTLEQWIEK RRGEKLDKVL ALELFEQITK GVDYIHSKKL IHRDLKPSNI
   421  FLVDTKQVKI GDFGLVTSLK NDGKRTRSKG TLRYMSPEQI SSQDYGKEVD LYALGLILAE
   481  LLHVCDTAFE TSKFFTDLRD GIISDIFDKK EKTLLQKLLS KKPEDRPNTS EILRTLTVWK
   541  KSPEKNERHT C

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EIF2AK2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.36
Highest tissue expression
29 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 29 nTPM
  • salivary gland: 17 nTPM
  • spleen: 11 nTPM
  • thymus: 11 nTPM
  • cerebral cortex: 11 nTPM
  • ovary: 11 nTPM

Single-cell type

  • neutrophils: 245 nCPM
  • neutrophil progenitors: 242 nCPM
  • extravillous trophoblasts: 173 nCPM
  • peritubular myoid cells: 136 nCPM
  • schwann cells: 128 nCPM
  • leydig cells: 125 nCPM

Immune cell

  • neutrophil: 16 nTPM
  • basophil: 11 nTPM
  • non-classical monocyte: 8 nTPM
  • intermediate monocyte: 7.9 nTPM
  • NK-cell: 6.5 nTPM
  • T-reg: 6.5 nTPM

Brain region

  • spinal cord: 49 nTPM
  • hypothalamus: 43 nTPM
  • medulla oblongata: 40 nTPM
  • pons: 39 nTPM
  • cerebral cortex: 35 nTPM
  • choroid plexus: 34 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about EIF2AK2.

Disease | AllUniProt

Conditions EIF2AK2 is implicated in, by any mechanism.

  • Leukoencephalopathy, developmental delay, and episodic neurologic regression syndrome (LEUDEN) MIM:618877
  • Dystonia 33 (DYT33) MIM:619687

Disease | GeneticClinVar

5 pathogenic / likely-pathogenic of 272 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Leukoencephalopathy, developmental delay, and episodic neurologic regression syndrome
  • Dystonia 33
  • See cases

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.52
gnomAD pLI
0.02
gnomAD missense Z
1.28
DepMap mean gene effect
0.15
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EIF2AK2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EIF2AK2 as an antibody target. Whether an autoantibody or antibody against EIF2AK2 could matter depends on whether native EIF2AK2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EIF2AK2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EIF2AK2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EIF2AK2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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