NCK1
SH2/SH3 adapter protein NCK1
Also known as: NCK, NCK1_HUMAN, NCKalpha
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P16333
- Gene
- NCK1
- Ensembl
- ENSG00000158092
- Chromosome
- 3
- Canonical length
- 377 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Plasma membrane,Cytosol
OverviewNCBI Gene
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]
Canonical amino-acid sequenceUniProt
377 residues, UniProt reviewed canonical sequence.
>P16333|NCK1
1 MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN
61 SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE
121 REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA
181 AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVI EKPENDPEWW KCRKINGMVG
241 LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG
301 HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME ELVEHYKKAP
361 IFTSEQGEKL YLVKHLSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NCK1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.4
- Highest tissue expression
- 49 nTPM
Expression across tissuesHPA
Tissue
- thymus: 49 nTPM
- tonsil: 42 nTPM
- lymph node: 39 nTPM
- esophagus: 36 nTPM
- vagina: 35 nTPM
- adipose tissue: 33 nTPM
Single-cell type
- microglia: 162 nCPM
- myonuclei: 148 nCPM
- adipocytes: 98 nCPM
- nk-cells: 97 nCPM
- hematopoietic stem cells: 97 nCPM
- t-cells: 97 nCPM
Immune cell
- basophil: 61 nTPM
- intermediate monocyte: 51 nTPM
- MAIT T-cell: 34 nTPM
- memory CD8 T-cell: 34 nTPM
- gdT-cell: 33 nTPM
- T-reg: 28 nTPM
Brain region
- choroid plexus: 43 nTPM
- cerebellum: 36 nTPM
- white matter: 33 nTPM
- medulla oblongata: 32 nTPM
- midbrain: 31 nTPM
- thalamus: 31 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.83
- gnomAD pLI
- 0
- gnomAD missense Z
- 1.27
- DepMap mean gene effect
- -0.02
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- actin filament organization
- antiviral innate immune response
- cell migration
- ephrin receptor signaling pathway
- lamellipodium assembly
- negative regulation of insulin receptor signaling pathway
- negative regulation of PERK-mediated unfolded protein response
- negative regulation of T cell receptor signaling pathway
- negative regulation of transcription by RNA polymerase II
- positive regulation of actin filament polymerization
- positive regulation of cap-dependent translational initiation
- positive regulation of cap-independent translational initiation
- positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
- positive regulation of neuron projection development
- positive regulation of T cell proliferation
- positive regulation of transcription by RNA polymerase II
- positive regulation of translation in response to endoplasmic reticulum stress
- regulation of cell migration
- regulation of translation initiation in response to endoplasmic reticulum stress
- response to endoplasmic reticulum stress
- signal complex assembly
- substrate-dependent cell migration, cell extension
- T cell activation
Molecular functions
- cadherin binding
- cytoskeletal anchor activity
- ephrin receptor binding
- eukaryotic initiation factor eIF2 binding
- molecular condensate scaffold activity
- protein domain specific binding
- protein kinase inhibitor activity
- protein sequestering activity
- protein-macromolecule adaptor activity
- receptor tyrosine kinase binding
- signaling adaptor activity
- signaling receptor binding
- signaling receptor complex adaptor activity
Cellular components
Protein domainsUniProt · Pfam · InterPro
- SH2 domain
- SH3 domain
- Cytoplasmic protein NCK
- SH3-like domain superfamily
- SH2 domain superfamily
- Tyrosine-phosphorylated adapter molecule
- SH2 domain
- SH3 domain
- Nck1, SH3 domain 1
- Nck1, SH3 domain 2
- Nck1, SH3 domain 3
- Nck1, SH2 domain
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of NCK1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NCK1 as an antibody target. Whether an autoantibody or antibody against NCK1 could matter depends on whether native NCK1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NCK1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label NCK1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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