Seroatlas · Human Serome Atlas

RPN1

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

Also known as: OST1, RPN1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P04843
Gene: RPN1
Ensembl: ENSG00000163902
Chromosome: 3
Canonical length: 607 aa
Protein class: Cancer-related genes, Metabolic proteins, Plasma proteins, Predicted membrane proteins, Transporters
Subcellular location: Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

607 residues, UniProt reviewed canonical sequence.

>P04843|RPN1
     1  MEAPAAGLFL LLLLGTWAPA PGSASSEAPP LINEDVKRTV DLSSHLAKVT AEVVLAHLGG
    61  GSTSRATSFL LALEPELEAR LAHLGVQVKG EDEEENNLEV RETKIKGKSG RFFTVKLPVA
   121  LDPGAKISVI VETVYTHVLH PYPTQITQSE KQFVVFEGNH YFYSPYPTKT QTMRVKLASR
   181  NVESYTKLGN PTRSEDLLDY GPFRDVPAYS QDTFKVHYEN NSPFLTITSM TRVIEVSHWG
   241  NIAVEENVDL KHTGAVLKGP FSRYDYQRQP DSGISSIRSF KTILPAAAQD VYYRDEIGNV
   301  STSHLLILDD SVEMEIRPRF PLFGGWKTHY IVGYNLPSYE YLYNLGDQYA LKMRFVDHVF
   361  DEQVIDSLTV KIILPEGAKN IEIDSPYEIS RAPDELHYTY LDTFGRPVIV AYKKNLVEQH
   421  IQDIVVHYTF NKVLMLQEPL LVVAAFYILF FTVIIYVRLD FSITKDPAAE ARMKVACITE
   481  QVLTLVNKRI GLYRHFDETV NRYKQSRDIS TLNSGKKSLE TEHKALTSEI ALLQSRLKTE
   541  GSDLCDRVSE MQKLDAQVKE LVLKSAVEAE RLVAGKLKKD TYIENEKLIS GKRQELVTKI
   601  DHILDAL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPN1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.3
Highest tissue expression: 333 nTPM

Expression across tissuesHPA

Tissue

epididymis: 333 nTPM
liver: 212 nTPM
choroid plexus: 169 nTPM
thyroid gland: 166 nTPM
parathyroid gland: 135 nTPM
cervix: 134 nTPM

Single-cell type

epididymal principal cells: 323 nCPM
kupffer cells: 311 nCPM
extravillous trophoblasts: 286 nCPM
plasma cells: 281 nCPM
syncytiotrophoblasts: 207 nCPM
hepatocytes: 204 nCPM

Immune cell

total PBMC: 251 nTPM
neutrophil: 234 nTPM
plasmacytoid DC: 193 nTPM
basophil: 178 nTPM
classical monocyte: 172 nTPM
non-classical monocyte: 160 nTPM

Brain region

choroid plexus: 151 nTPM
white matter: 112 nTPM
medulla oblongata: 103 nTPM
hypothalamus: 99 nTPM
thalamus: 94 nTPM
cerebellum: 93 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.48
gnomAD pLI: 0.24
gnomAD missense Z: 0.74
DepMap mean gene effect: -0.97
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

RNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

Ribophorin I
Ribophorin I

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPN1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPN1 as an antibody target. Whether an autoantibody or antibody against RPN1 could matter depends on whether native RPN1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPN1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPN1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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