TMEM35A
Novel acetylcholine receptor chaperone
Also known as: FLJ14084, NACHO, NACHO_HUMAN, TMEM35, TUF-1
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q53FP2
- Gene
- TMEM35A
- Ensembl
- ENSG00000126950
- Chromosome
- X
- Canonical length
- 167 aa
- Protein class
- Predicted membrane proteins, Transporters
- Subcellular location
- Vesicles,Focal adhesion sites,Cytosol
OverviewNCBI Gene
Enables acetylcholine receptor regulator activity. Involved in chaperone-mediated protein complex assembly and positive regulation of protein localization to cell surface. Located in endoplasmic reticulum. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
167 residues, UniProt reviewed canonical sequence.
>Q53FP2|TMEM35A
1 MASPRTVTIV ALSVALGLFF VFMGTIKLTP RLSKDAYSEM KRAYKSYVRA LPLLKKMGIN
61 SILLRKSIGA LEVACGIVMT LVPGRPKDVA NFFLLLLVLA VLFFHQLVGD PLKRYAHALV
121 FGILLTCRLL IARKPEDRSS EKKPLPGNAE EQPSLYEKAP QGKVKVSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against TMEM35A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Other membrane
- Secreted
- No
- Transmembrane segments
- 4
- Mean surface accessibility (rSASA)
- 0.4
- Highest tissue expression
- 56 nTPM
Expression across tissuesHPA
Tissue
- hypothalamus: 56 nTPM
- seminal vesicle: 48 nTPM
- basal ganglia: 42 nTPM
- cerebellum: 38 nTPM
- colon: 37 nTPM
- cerebral cortex: 32 nTPM
Single-cell type
- other brain neurons: 63 nCPM
- brain inhibitory neurons: 42 nCPM
- decidual stromal cells: 37 nCPM
- smooth muscle cells: 34 nCPM
- brain excitatory neurons: 34 nCPM
- oligodendrocyte progenitor cells: 25 nCPM
Immune cell
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
- intermediate monocyte: 0 nTPM
- MAIT T-cell: 0 nTPM
Brain region
- hypothalamus: 157 nTPM
- pons: 80 nTPM
- hippocampal formation: 60 nTPM
- cerebral cortex: 60 nTPM
- medulla oblongata: 47 nTPM
- midbrain: 45 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.85
- gnomAD pLI
- 0.66
- DepMap mean gene effect
- 0.18
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- chaperone-mediated protein complex assembly
- positive regulation of protein localization to cell surface
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of TMEM35A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads TMEM35A as an antibody target. Whether an autoantibody or antibody against TMEM35A could matter depends on whether native TMEM35A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
TMEM35A is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label TMEM35A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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