Seroatlas · Human Serome Atlas

CANX

Calnexin

Also known as: CALX_HUMAN, CNX, IP90, P90

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P27824
Gene: CANX
Ensembl: ENSG00000127022
Chromosome: 5
Canonical length: 592 aa
Protein class: Cancer-related genes, Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location: Endoplasmic reticulum

OverviewNCBI Gene

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]

Canonical amino-acid sequenceUniProt

592 residues, UniProt reviewed canonical sequence.

>P27824|CANX
     1  MEGKWLLCML LVLGTAIVEA HDGHDDDVID IEDDLDDVIE EVEDSKPDTT APPSSPKVTY
    61  KAPVPTGEVY FADSFDRGTL SGWILSKAKK DDTDDEIAKY DGKWEVEEMK ESKLPGDKGL
   121  VLMSRAKHHA ISAKLNKPFL FDTKPLIVQY EVNFQNGIEC GGAYVKLLSK TPELNLDQFH
   181  DKTPYTIMFG PDKCGEDYKL HFIFRHKNPK TGIYEEKHAK RPDADLKTYF TDKKTHLYTL
   241  ILNPDNSFEI LVDQSVVNSG NLLNDMTPPV NPSREIEDPE DRKPEDWDER PKIPDPEAVK
   301  PDDWDEDAPA KIPDEEATKP EGWLDDEPEY VPDPDAEKPE DWDEDMDGEW EAPQIANPRC
   361  ESAPGCGVWQ RPVIDNPNYK GKWKPPMIDN PSYQGIWKPR KIPNPDFFED LEPFRMTPFS
   421  AIGLELWSMT SDIFFDNFII CADRRIVDDW ANDGWGLKKA ADGAAEPGVV GQMIEAAEER
   481  PWLWVVYILT VALPVFLVIL FCCSGKKQTS GMEYKKTDAP QPDVKEEEEE KEEEKDKGDE
   541  EEEGEEKLEE KQKSDAEEDG GTVSQEEEDR KPKAEEDEIL NRSPRNRKPR RE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CANX can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 163 nTPM

Expression across tissuesHPA

Tissue

pancreas: 163 nTPM
epididymis: 162 nTPM
liver: 157 nTPM
blood vessel: 143 nTPM
thyroid gland: 142 nTPM
hypothalamus: 136 nTPM

Single-cell type

late spermatids: 1,223 nCPM
early spermatids: 213 nCPM
salivary acinar cells: 167 nCPM
ependymal cells: 159 nCPM
plasma cells: 151 nCPM
megakaryocyte progenitors: 131 nCPM

Immune cell

total PBMC: 74 nTPM
classical monocyte: 62 nTPM
basophil: 52 nTPM
myeloid DC: 48 nTPM
intermediate monocyte: 39 nTPM
plasmacytoid DC: 39 nTPM

Brain region

choroid plexus: 77 nTPM
medulla oblongata: 74 nTPM
midbrain: 73 nTPM
hypothalamus: 71 nTPM
pons: 67 nTPM
white matter: 66 nTPM

ReferencesPubMed · IEDB

Publications for CANX from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

2 publications

Antibodies to the endoplasmic reticulum-resident chaperones calnexin, BiP and Grp94 in patients with rheumatoid arthritis and systemic lupus erythematosus.
2010 · Rheumatology (Oxford) · RCR 1 · 43 citations
Antibodies to calnexin and mutated calreticulin are common in human sera.
2023 · Curr Res Transl Med · RCR 0.5 · 4 citations

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.36
gnomAD pLI: 0.87
gnomAD missense Z: 1.58
DepMap mean gene effect: -0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CANX in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CANX as an antibody target. Whether an autoantibody or antibody against CANX could matter depends on whether native CANX is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CANX is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CANX as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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