DAD1

Protein identityUniProt · HPA

UniProt accession

P61803

Gene

DAD1

Ensembl

ENSG00000129562

Chromosome

14

Canonical length

113 aa

Protein class

Cancer-related genes, Metabolic proteins, Predicted membrane proteins, Transporters

Subcellular location

Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

113 residues, UniProt reviewed canonical sequence.

>P61803|DAD1
     1  MSASVVSVIS RFLEEYLSST PQRLKLLDAY LLYILLTGAL QFGYCLLVGT FPFNSFLSGF
    61  ISCVGSFILA VCLRIQINPQ NKADFQGISP ERAFADFLFA STILHLVVMN FVG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DAD1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

3

Mean surface accessibility (rSASA)

0.39

Highest tissue expression

454 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.37

gnomAD pLI

0.09

gnomAD missense Z

0.68

DepMap mean gene effect

-1.88

DepMap dependency class

pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic process
• blastocyst development
• glycoprotein biosynthetic process
• negative regulation of apoptotic process
• protein N-linked glycosylation
• protein N-linked glycosylation via asparagine
• regulation of protein stability

Molecular functions

• enzyme activator activity

Cellular components

• endoplasmic reticulum membrane
• membrane
• oligosaccharyltransferase complex
• oligosaccharyltransferase complex A
• oligosaccharyltransferase complex B

Protein domainsUniProt · Pfam · InterPro

• DAD/Ost2
• DAD family

KeywordsUniProt

• Acetylation
• Apoptosis
• Endoplasmic reticulum
• Membrane
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of DAD1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DDOST
• FAM241A
• MAGT1
• RPN1
• RPN2