Seroatlas · Human Serome Atlas

DDOST

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Also known as: KIAA0115, OST, OST48, OST48_HUMAN, WBP1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P39656
Gene
DDOST
Ensembl
ENSG00000244038
Chromosome
1
Canonical length
456 aa
Protein class
Disease related genes, Human disease related genes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location
Endoplasmic reticulum

OverviewNCBI Gene

This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

456 residues, UniProt reviewed canonical sequence.

>P39656|DDOST
     1  MGYFRCARAG SFGRRRKMEP STAARAWALF WLLLPLLGAV CASGPRTLVL LDNLNVRETH
    61  SLFFRSLKDR GFELTFKTAD DPSLSLIKYG EFLYDNLIIF SPSVEDFGGN INVETISAFI
   121  DGGGSVLVAA SSDIGDPLRE LGSECGIEFD EEKTAVIDHH NYDISDLGQH TLIVADTENL
   181  LKAPTIVGKS SLNPILFRGV GMVADPDNPL VLDILTGSST SYSFFPDKPI TQYPHAVGKN
   241  TLLIAGLQAR NNARVIFSGS LDFFSDSFFN SAVQKAAPGS QRYSQTGNYE LAVALSRWVF
   301  KEEGVLRVGP VSHHRVGETA PPNAYTVTDL VEYSIVIQQL SNGKWVPFDG DDIQLEFVRI
   361  DPFVRTFLKK KGGKYSVQFK LPDVYGVFQF KVDYNRLGYT HLYSSTQVSV RPLQHTQYER
   421  FIPSAYPYYA SAFSMMLGLF IFSIVFLHMK EKEKSD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDOST can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.32
Highest tissue expression
252 nTPM

Expression across tissuesHPA

Tissue

  • pancreas: 252 nTPM
  • epididymis: 212 nTPM
  • bone marrow: 181 nTPM
  • thyroid gland: 174 nTPM
  • placenta: 172 nTPM
  • spleen: 159 nTPM

Single-cell type

  • cytotrophoblasts: 404 nCPM
  • syncytiotrophoblasts: 401 nCPM
  • extravillous trophoblasts: 371 nCPM
  • migrating cytotrophoblasts: 368 nCPM
  • plasma cells: 320 nCPM
  • decidual stromal cells: 240 nCPM

Immune cell

  • total PBMC: 491 nTPM
  • myeloid DC: 391 nTPM
  • plasmacytoid DC: 321 nTPM
  • intermediate monocyte: 285 nTPM
  • basophil: 273 nTPM
  • MAIT T-cell: 271 nTPM

Brain region

  • choroid plexus: 88 nTPM
  • hypothalamus: 67 nTPM
  • thalamus: 66 nTPM
  • white matter: 61 nTPM
  • medulla oblongata: 55 nTPM
  • pons: 52 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DDOST.

Disease | AllUniProt

Conditions DDOST is implicated in, by any mechanism.

  • Congenital disorder of glycosylation 1R (CDG1R) MIM:614507

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 318 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Congenital disorder of glycosylation type Ir

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.73
gnomAD pLI
0
gnomAD missense Z
0.79
DepMap mean gene effect
-1.02
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48kDa subunit
  • OST48, N-terminal domain
  • OST48, middle domain
  • OST48, N-terminal domain
  • OST48 middle domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DDOST in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DDOST as an antibody target. Whether an autoantibody or antibody against DDOST could matter depends on whether native DDOST is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DDOST is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DDOST as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DDOST. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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