Seroatlas · Human Serome Atlas

EMC8

ER membrane protein complex subunit 8

Also known as: C16orf2, C16orf4, COX4NB, EMC8_HUMAN, FAM158B, NOC4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O43402
Gene: EMC8
Ensembl: ENSG00000131148
Chromosome: 16
Canonical length: 210 aa
Protein class: Predicted intracellular proteins, Transporters
Subcellular location: Endoplasmic reticulum,Golgi apparatus

OverviewNCBI Gene

Contributes to membrane insertase activity. Involved in protein insertion into ER membrane by stop-transfer membrane-anchor sequence and tail-anchored membrane protein insertion into ER membrane. Located in Golgi apparatus and endoplasmic reticulum. Part of EMC complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

210 residues, UniProt reviewed canonical sequence.

>O43402|EMC8
     1  MPGVKLTTQA YCKMVLHGAK YPHCAVNGLL VAEKQKPRKE HLPLGGPGAH HTLFVDCIPL
    61  FHGTLALAPM LEVALTLIDS WCKDHSYVIA GYYQANERVK DASPNQVAEK VASRIAEGFS
   121  DTALIMVDNT KFTMDCVAPT IHVYEHHENR WRCRDPHHDY CEDWPEAQRI SASLLDSRSY
   181  ETLVDFDNHL DDIRNDWTNP EINKAVLHLC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EMC8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 20 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 20 nTPM
heart muscle: 18 nTPM
bone marrow: 16 nTPM
basal ganglia: 14 nTPM
adipose tissue: 13 nTPM
amygdala: 13 nTPM

Single-cell type

early spermatids: 248 nCPM
late primary spermatocytes: 187 nCPM
esophageal basal cells: 74 nCPM
endometrial glandular cells: 72 nCPM
extravillous trophoblasts: 72 nCPM
hofbauer cells: 63 nCPM

Immune cell

eosinophil: 26 nTPM
myeloid DC: 24 nTPM
non-classical monocyte: 20 nTPM
intermediate monocyte: 19 nTPM
NK-cell: 19 nTPM
classical monocyte: 18 nTPM

Brain region

white matter: 16 nTPM
spinal cord: 14 nTPM
thalamus: 14 nTPM
medulla oblongata: 14 nTPM
cerebellum: 13 nTPM
basal ganglia: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.45
gnomAD pLI: 0.85
gnomAD missense Z: 0.62
DepMap mean gene effect: -0.19
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EMC8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EMC8 as an antibody target. Whether an autoantibody or antibody against EMC8 could matter depends on whether native EMC8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EMC8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EMC8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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