Seroatlas · Human Serome Atlas

DNAJB12

DnaJ homolog subfamily B member 12

Also known as: DJ10, DJB12_HUMAN, FLJ20027

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NXW2
Gene
DNAJB12
Ensembl
ENSG00000148719
Chromosome
10
Canonical length
375 aa
Protein class
Predicted membrane proteins, Transporters
Subcellular location
Nuclear membrane,Endoplasmic reticulum
Quaternary structure
Homotetramer

OverviewNCBI Gene

DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

375 residues, UniProt reviewed canonical sequence.

>Q9NXW2|DNAJB12
     1  MESNKDEAER CISIALKAIQ SNQPDRALRF LEKAQRLYPT PRVRALIESL NQKPQTAGDQ
    61  PPPTDTTHAT HRKAGGTDAP SANGEAGGES TKGYTAEQVA AVKRVKQCKD YYEILGVSRG
   121  ASDEDLKKAY RRLALKFHPD KNHAPGATEA FKAIGTAYAV LSNPEKRKQY DQFGDDKSQA
   181  ARHGHGHGDF HRGFEADISP EDLFNMFFGG GFPSSNVHVY SNGRMRYTYQ QRQDRRDNQG
   241  DGGLGVFVQL MPILILILVS ALSQLMVSSP PYSLSPRPSV GHIHRRVTDH LGVVYYVGDT
   301  FSEEYTGSSL KTVERNVEDD YIANLRNNCW KEKQQKEGLL YRARYFGDTD MYHRAQKMGT
   361  PSCSRLSEVQ ASLHG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAJB12 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.45
Highest tissue expression
37 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 37 nTPM
  • parathyroid gland: 31 nTPM
  • adrenal gland: 30 nTPM
  • skin: 29 nTPM
  • liver: 28 nTPM
  • salivary gland: 27 nTPM

Single-cell type

  • late spermatids: 347 nCPM
  • early spermatids: 120 nCPM
  • late primary spermatocytes: 104 nCPM
  • breast lactating cells: 94 nCPM
  • syncytiotrophoblasts: 87 nCPM
  • epididymal principal cells: 85 nCPM

Immune cell

  • neutrophil: 37 nTPM
  • intermediate monocyte: 32 nTPM
  • classical monocyte: 29 nTPM
  • non-classical monocyte: 29 nTPM
  • myeloid DC: 25 nTPM
  • basophil: 19 nTPM

Brain region

  • medulla oblongata: 30 nTPM
  • cerebellum: 29 nTPM
  • cerebral cortex: 29 nTPM
  • choroid plexus: 29 nTPM
  • white matter: 29 nTPM
  • basal ganglia: 28 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.49
gnomAD pLI
0.44
gnomAD missense Z
0.58
DepMap mean gene effect
-0.25
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DNAJB12 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNAJB12 as an antibody target. Whether an autoantibody or antibody against DNAJB12 could matter depends on whether native DNAJB12 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNAJB12 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNAJB12 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DNAJB12. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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