Seroatlas · Human Serome Atlas

KPNA1

Importin subunit alpha-5

Also known as: IMA5_HUMAN, IPOA5, NPI-1, RCH2, SRP1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P52294
Gene
KPNA1
Ensembl
ENSG00000114030
Chromosome
3
Canonical length
538 aa
Protein class
Predicted intracellular proteins, Transporters
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Canonical amino-acid sequenceUniProt

538 residues, UniProt reviewed canonical sequence.

>P52294|KPNA1
     1  MTTPGKENFR LKSYKNKSLN PDEMRRRREE EGLQLRKQKR EEQLFKRRNV ATAEEETEEE
    61  VMSDGGFHEA QISNMEMAPG GVITSDMIEM IFSKSPEQQL SATQKFRKLL SKEPNPPIDE
   121  VISTPGVVAR FVEFLKRKEN CTLQFESAWV LTNIASGNSL QTRIVIQAGA VPIFIELLSS
   181  EFEDVQEQAV WALGNIAGDS TMCRDYVLDC NILPPLLQLF SKQNRLTMTR NAVWALSNLC
   241  RGKSPPPEFA KVSPCLNVLS WLLFVSDTDV LADACWALSY LSDGPNDKIQ AVIDAGVCRR
   301  LVELLMHNDY KVVSPALRAV GNIVTGDDIQ TQVILNCSAL QSLLHLLSSP KESIKKEACW
   361  TISNITAGNR AQIQTVIDAN IFPALISILQ TAEFRTRKEA AWAITNATSG GSAEQIKYLV
   421  ELGCIKPLCD LLTVMDSKIV QVALNGLENI LRLGEQEAKR NGTGINPYCA LIEEAYGLDK
   481  IEFLQSHENQ EIYQKAFDLI EHYFGTEDED SSIAPQVDLN QQQYIFQQCE APMEGFQL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against KPNA1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
66 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 66 nTPM
  • tongue: 38 nTPM
  • liver: 30 nTPM
  • retina: 27 nTPM
  • cerebral cortex: 22 nTPM
  • smooth muscle: 21 nTPM

Single-cell type

  • neutrophils: 418 nCPM
  • late spermatids: 306 nCPM
  • myonuclei: 222 nCPM
  • neutrophil progenitors: 215 nCPM
  • rod photoreceptor cells: 174 nCPM
  • endometrial ciliated cells: 171 nCPM

Immune cell

  • neutrophil: 42 nTPM
  • non-classical monocyte: 30 nTPM
  • basophil: 27 nTPM
  • myeloid DC: 26 nTPM
  • NK-cell: 21 nTPM
  • intermediate monocyte: 20 nTPM

Brain region

  • cerebral cortex: 43 nTPM
  • white matter: 38 nTPM
  • spinal cord: 37 nTPM
  • medulla oblongata: 36 nTPM
  • thalamus: 36 nTPM
  • pons: 36 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.25
gnomAD pLI
1
gnomAD missense Z
3.2
DepMap mean gene effect
0.03
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 12% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of KPNA1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads KPNA1 as an antibody target. Whether an autoantibody or antibody against KPNA1 could matter depends on whether native KPNA1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

KPNA1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label KPNA1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/KPNA1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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