Seroatlas · Human Serome Atlas

ALKBH5

RNA demethylase ALKBH5

Also known as: ALKB5_HUMAN, FLJ20308, OFOXD1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6P6C2
Gene
ALKBH5
Ensembl
ENSG00000091542
Chromosome
17
Canonical length
394 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Golgi apparatus,Cytosol

OverviewNCBI Gene

Enables mRNA N6-methyladenosine dioxygenase activity and molecular condensate scaffold activity. Involved in membraneless organelle assembly; post-transcriptional regulation of gene expression; and response to hypoxia. Acts upstream of or within regulation of mRNA export from nucleus and regulation of mRNA processing. Located in Golgi apparatus; cytosol; and nuclear speck. Is active in paraspeckles. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

394 residues, UniProt reviewed canonical sequence.

>Q6P6C2|ALKBH5
     1  MAAASGYTDL REKLKSMTSR DNYKAGSREA AAAAAAAVAA AAAAAAAAEP YPVSGAKRKY
    61  QEDSDPERSD YEEQQLQKEE EARKVKSGIR QMRLFSQDEC AKIEARIDEV VSRAEKGLYN
   121  EHTVDRAPLR NKYFFGEGYT YGAQLQKRGP GQERLYPPGD VDEIPEWVHQ LVIQKLVEHR
   181  VIPEGFVNSA VINDYQPGGC IVSHVDPIHI FERPIVSVSF FSDSALCFGC KFQFKPIRVS
   241  EPVLSLPVRR GSVTVLSGYA ADEITHCIRP QDIKERRAVI ILRKTRLDAP RLETKSLSSS
   301  VLPPSYASDR LSGNNRDPAL KPKRSHRKAD PDAAHRPRIL EMDKEENRRS VLLPTHRRRG
   361  SFSSENYWRK SYESSEDCSE AAGSPARKVK MRRH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ALKBH5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.44
Highest tissue expression
222 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 222 nTPM
  • tongue: 92 nTPM
  • heart muscle: 60 nTPM
  • testis: 41 nTPM
  • blood vessel: 40 nTPM
  • fallopian tube: 38 nTPM

Single-cell type

  • early primary spermatocytes: 126 nCPM
  • undifferentiated spermatogonia: 123 nCPM
  • fallopian tube ciliated cells: 119 nCPM
  • retinal horizontal cells: 118 nCPM
  • differentiating spermatogonia: 109 nCPM
  • müller glia: 107 nCPM

Immune cell

  • eosinophil: 20 nTPM
  • memory CD8 T-cell: 16 nTPM
  • memory CD4 T-cell: 13 nTPM
  • gdT-cell: 13 nTPM
  • MAIT T-cell: 12 nTPM
  • non-classical monocyte: 12 nTPM

Brain region

  • pons: 139 nTPM
  • thalamus: 134 nTPM
  • white matter: 129 nTPM
  • cerebral cortex: 127 nTPM
  • midbrain: 126 nTPM
  • hypothalamus: 124 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.34
gnomAD pLI
0.95
gnomAD missense Z
2.56
DepMap mean gene effect
-0.14
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ALKBH5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ALKBH5 as an antibody target. Whether an autoantibody or antibody against ALKBH5 could matter depends on whether native ALKBH5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ALKBH5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ALKBH5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ALKBH5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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