ACSS2

Protein identityUniProt · HPA

UniProt accession

Q9NR19

Gene

ACSS2

Ensembl

ENSG00000131069

Chromosome

20

Canonical length

701 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]

Canonical amino-acid sequenceUniProt

701 residues, UniProt reviewed canonical sequence.

>Q9NR19|ACSS2
     1  MGLPEERVRS GSGSRGQEEA GAGGRARSWS PPPEVSRSAH VPSLQRYREL HRRSVEEPRE
    61  FWGDIAKEFY WKTPCPGPFL RYNFDVTKGK IFIEWMKGAT TNICYNVLDR NVHEKKLGDK
   121  VAFYWEGNEP GETTQITYHQ LLVQVCQFSN VLRKQGIQKG DRVAIYMPMI PELVVAMLAC
   181  ARIGALHSIV FAGFSSESLC ERILDSSCSL LITTDAFYRG EKLVNLKELA DEALQKCQEK
   241  GFPVRCCIVV KHLGRAELGM GDSTSQSPPI KRSCPDVQIS WNQGIDLWWH ELMQEAGDEC
   301  EPEWCDAEDP LFILYTSGST GKPKGVVHTV GGYMLYVATT FKYVFDFHAE DVFWCTADIG
   361  WITGHSYVTY GPLANGATSV LFEGIPTYPD VNRLWSIVDK YKVTKFYTAP TAIRLLMKFG
   421  DEPVTKHSRA SLQVLGTVGE PINPEAWLWY HRVVGAQRCP IVDTFWQTET GGHMLTPLPG
   481  ATPMKPGSAT FPFFGVAPAI LNESGEELEG EAEGYLVFKQ PWPGIMRTVY GNHERFETTY
   541  FKKFPGYYVT GDGCQRDQDG YYWITGRIDD MLNVSGHLLS TAEVESALVE HEAVAEAAVV
   601  GHPHPVKGEC LYCFVTLCDG HTFSPKLTEE LKKQIREKIG PIATPDYIQN APGLPKTRSG
   661  KIMRRVLRKI AQNDHDLGDM STVADPSVIS HLFSHRCLTI Q

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACSS2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.21

Highest tissue expression

110 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.9

gnomAD pLI

0

gnomAD missense Z

1.08

DepMap mean gene effect

0.07

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• acetate biosynthetic process
• acetyl-CoA biosynthetic process
• acetyl-CoA biosynthetic process from acetate
• ethanol catabolic process
• lipid biosynthetic process
• long-term memory
• propionate biosynthetic process

Molecular functions

• acetate-CoA ligase activity
• AMP binding
• ATP binding
• chromatin binding
• propionate-CoA ligase activity
• transcription coactivator activity

Cellular components

• cytoplasm
• cytosol
• mitochondrial matrix
• nucleus

Protein domainsUniProt · Pfam · InterPro

• AMP-dependent synthetase/ligase domain
• Acetate-CoA ligase
• AMP-binding, conserved site
• AMP-binding enzyme, C-terminal domain
• Acetyl-coenzyme A synthetase, N-terminal domain
• ANL, N-terminal domain
• AMP-binding enzyme domain superfamily
• AMP-binding enzyme
• AMP-binding enzyme C-terminal domain
• Acetyl-coenzyme A synthetase N-terminus

KeywordsUniProt

• Acetylation
• Activator
• ATP-binding
• Cytoplasm
• Ligase
• Lipid metabolism
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of ACSS2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CREBBP
• KPNA1
• TFEB
• PRKAA2