SSRP1

Protein identityUniProt · HPA

UniProt accession

Q08945

Gene

SSRP1

Ensembl

ENSG00000149136

Chromosome

11

Canonical length

709 aa

Protein class

Plasma proteins, Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

709 residues, UniProt reviewed canonical sequence.

>Q08945|SSRP1
     1  MAETLEFNDV YQEVKGSMND GRLRLSRQGI IFKNSKTGKV DNIQAGELTE GIWRRVALGH
    61  GLKLLTKNGH VYKYDGFRES EFEKLSDFFK THYRLELMEK DLCVKGWNWG TVKFGGQLLS
   121  FDIGDQPVFE IPLSNVSQCT TGKNEVTLEF HQNDDAEVSL MEVRFYVPPT QEDGVDPVEA
   181  FAQNVLSKAD VIQATGDAIC IFRELQCLTP RGRYDIRIYP TFLHLHGKTF DYKIPYTTVL
   241  RLFLLPHKDQ RQMFFVISLD PPIKQGQTRY HFLILLFSKD EDISLTLNMN EEEVEKRFEG
   301  RLTKNMSGSL YEMVSRVMKA LVNRKITVPG NFQGHSGAQC ITCSYKASSG LLYPLERGFI
   361  YVHKPPVHIR FDEISFVNFA RGTTTTRSFD FEIETKQGTQ YTFSSIEREE YGKLFDFVNA
   421  KKLNIKNRGL KEGMNPSYDE YADSDEDQHD AYLERMKEEG KIREENANDS SDDSGEETDE
   481  SFNPGEEEED VAEEFDSNAS ASSSSNEGDS DRDEKKRKQL KKAKMAKDRK SRKKPVEVKK
   541  GKDPNAPKRP MSAYMLWLNA SREKIKSDHP GISITDLSKK AGEIWKGMSK EKKEEWDRKA
   601  EDARRDYEKA MKEYEGGRGE SSKRDKSKKK KKVKVKMEKK STPSRGSSSK SSSRQLSESF
   661  KSKEFVSSDE SSSGENKSKK KRRRSEDSEE EELASTPPSS EDSASGSDE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SSRP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.42

Highest tissue expression

134 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for SSRP1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.28

gnomAD pLI

1

gnomAD missense Z

4.04

DepMap mean gene effect

-1.31

DepMap dependency class

pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• DNA repair
• DNA replication
• nucleosome assembly
• nucleosome disassembly
• regulation of chromatin organization

Molecular functions

• DNA binding
• histone binding
• nucleosome binding
• RNA binding

Cellular components

• FACT complex
• nucleolus
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• High mobility group box domain
• PH-like domain superfamily
• Histone chaperone RTT106/FACT complex subunit SPT16-like, middle domain
• High mobility group box domain superfamily
• HMG (high mobility group) box
• Histone chaperone Rttp106-like, middle domain
• FACT complex subunit SSRP1/POB3
• SSRP1, dimerization domain
• FACT complex subunit SSRP1/POB3, N-terminal PH domain
• SSRP1 domain superfamily
• FACT complex subunit SSRP1, C-terminal domain
• FACT complex subunit SSRP1-like, first PH domain
• RTT106/SSRP1 Histone Chaperone/FACT Complex
• Structure-specific recognition protein (SSRP1)
• POB3-like N-terminal PH domain
• FACT complex subunit SSRP1, C-terminal domain
• SSRP1 PH domain

KeywordsUniProt

• Acetylation
• Chromosome
• DNA damage
• DNA repair
• DNA replication
• DNA-binding
• Host-virus interaction
• Isopeptide bond
• Nucleus
• Phosphoprotein
• Transcription
• Transcription regulation
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of SSRP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MYOG
• NEK9
• SRF
• TP63
• CHD1
• FYTTD1
• ACIN1
• ATP2B1
• BRD7
• CBX3
• CHD4
• CHMP4C
• CSNK2A1
• CSNK2A2
• CSNK2B
• CTR9
• DDB1
• DDX21
• DHX9
• EED
• EFTUD2
• GINS3
• H2AC21
• H2AX
• H2BC1
• H2BC21
• H2BC26
• HNRNPC
• HNRNPD
• HNRNPR
• LEO1
• MACROH2A2
• MCM2
• MCM4
• MCM6
• MCM7
• MEN1
• MMS22L
• NAA40
• PAF1