Seroatlas · Human Serome Atlas

MCM2

DNA replication licensing factor MCM2

Also known as: BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030, MCM2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P49736
Gene: MCM2
Ensembl: ENSG00000073111
Chromosome: 3
Canonical length: 904 aa
Protein class: Cancer-related genes, Disease related genes, Enzymes, Human disease related genes, Plasma proteins, Potential drug targets, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Vesicles,Primary cilium,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Canonical amino-acid sequenceUniProt

904 residues, UniProt reviewed canonical sequence.

>P49736|MCM2
     1  MAESSESFTM ASSPAQRRRG NDPLTSSPGR SSRRTDALTS SPGRDLPPFE DESEGLLGTE
    61  GPLEEEEDGE ELIGDGMERD YRAIPELDAY EAEGLALDDE DVEELTASQR EAAERAMRQR
   121  DREAGRGLGR MRRGLLYDSD EEDEERPARK RRQVERATED GEEDEEMIES IENLEDLKGH
   181  SVREWVSMAG PRLEIHHRFK NFLRTHVDSH GHNVFKERIS DMCKENRESL VVNYEDLAAR
   241  EHVLAYFLPE APAELLQIFD EAALEVVLAM YPKYDRITNH IHVRISHLPL VEELRSLRQL
   301  HLNQLIRTSG VVTSCTGVLP QLSMVKYNCN KCNFVLGPFC QSQNQEVKPG SCPECQSAGP
   361  FEVNMEETIY QNYQRIRIQE SPGKVAAGRL PRSKDAILLA DLVDSCKPGD EIELTGIYHN
   421  NYDGSLNTAN GFPVFATVIL ANHVAKKDNK VAVGELTDED VKMITSLSKD QQIGEKIFAS
   481  IAPSIYGHED IKRGLALALF GGEPKNPGGK HKVRGDINVL LCGDPGTAKS QFLKYIEKVS
   541  SRAIFTTGQG ASAVGLTAYV QRHPVSREWT LEAGALVLAD RGVCLIDEFD KMNDQDRTSI
   601  HEAMEQQSIS ISKAGIVTSL QARCTVIAAA NPIGGRYDPS LTFSENVDLT EPIISRFDIL
   661  CVVRDTVDPV QDEMLARFVV GSHVRHHPSN KEEEGLANGS AAEPAMPNTY GVEPLPQEVL
   721  KKYIIYAKER VHPKLNQMDQ DKVAKMYSDL RKESMATGSI PITVRHIESM IRMAEAHARI
   781  HLRDYVIEDD VNMAIRVMLE SFIDTQKFSV MRSMRKTFAR YLSFRRDNNE LLLFILKQLV
   841  AEQVTYQRNR FGAQQDTIEV PEKDLVDKAR QINIHNLSAF YDSELFRMNK FSHDLKRKMI
   901  LQQF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MCM2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.36
Highest tissue expression: 64 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 64 nTPM
thymus: 35 nTPM
lymph node: 25 nTPM
tonsil: 24 nTPM
placenta: 16 nTPM
appendix: 16 nTPM

Single-cell type

erythrocyte progenitors: 122 nCPM
cytotrophoblasts: 77 nCPM
megakaryocyte progenitors: 63 nCPM
migrating cytotrophoblasts: 59 nCPM
monocyte progenitors: 44 nCPM
megakaryocyte-erythroid progenitors: 38 nCPM

Immune cell

T-reg: 11 nTPM
memory B-cell: 9.7 nTPM
naive B-cell: 8.1 nTPM
NK-cell: 6.5 nTPM
MAIT T-cell: 5.5 nTPM
memory CD8 T-cell: 5.4 nTPM

Brain region

pons: 5.2 nTPM
cerebellum: 5.1 nTPM
white matter: 5 nTPM
medulla oblongata: 4.2 nTPM
hypothalamus: 4.1 nTPM
spinal cord: 4.1 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MCM2.

Disease | AllUniProt

Conditions MCM2 is implicated in, by any mechanism.

Deafness, autosomal dominant, 70 (DFNA70) MIM:616968

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.59
gnomAD pLI: 0
gnomAD missense Z: 1.68
DepMap mean gene effect: -1.58
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

MCM domain
Nucleic acid-binding, OB-fold
Mini-chromosome maintenance, conserved site
P-loop containing nucleoside triphosphate hydrolase
MCM, N-terminal domain
Mini-chromosome maintenance protein
MCM OB domain
MCM, AAA-lid domain
MCM P-loop domain
MCM N-terminal domain
MCM OB domain
MCM AAA-lid domain
DNA replication licensing factor Mcm2
DNA replication licensing factor MCM2-like, winged-helix domain
Mini-chromosome maintenance protein 2
DNA replication licensing factor MCM2-like, winged-helix domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MCM2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MCM2 as an antibody target. Whether an autoantibody or antibody against MCM2 could matter depends on whether native MCM2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MCM2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MCM2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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