Seroatlas · Human Serome Atlas

VPS35

Vacuolar protein sorting-associated protein 35

Also known as: FLJ10752, MEM3, PARK17, VPS35_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96QK1
Gene
VPS35
Ensembl
ENSG00000069329
Chromosome
16
Canonical length
796 aa
Protein class
Disease related genes, Human disease related genes, Plasma proteins, Potential drug targets, Predicted intracellular proteins, Transporters
Subcellular location
Endosomes,Lysosomes

OverviewNCBI Gene

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

796 residues, UniProt reviewed canonical sequence.

>Q96QK1|VPS35
     1  MPTTQQSPQD EQEKLLDEAI QAVKVQSFQM KRCLDKNKLM DALKHASNML GELRTSMLSP
    61  KSYYELYMAI SDELHYLEVY LTDEFAKGRK VADLYELVQY AGNIIPRLYL LITVGVVYVK
   121  SFPQSRKDIL KDLVEMCRGV QHPLRGLFLR NYLLQCTRNI LPDEGEPTDE ETTGDISDSM
   181  DFVLLNFAEM NKLWVRMQHQ GHSRDREKRE RERQELRILV GTNLVRLSQL EGVNVERYKQ
   241  IVLTGILEQV VNCRDALAQE YLMECIIQVF PDEFHLQTLN PFLRACAELH QNVNVKNIII
   301  ALIDRLALFA HREDGPGIPA DIKLFDIFSQ QVATVIQSRQ DMPSEDVVSL QVSLINLAMK
   361  CYPDRVDYVD KVLETTVEIF NKLNLEHIAT SSAVSKELTR LLKIPVDTYN NILTVLKLKH
   421  FHPLFEYFDY ESRKSMSCYV LSNVLDYNTE IVSQDQVDSI MNLVSTLIQD QPDQPVEDPD
   481  PEDFADEQSL VGRFIHLLRS EDPDQQYLIL NTARKHFGAG GNQRIRFTLP PLVFAAYQLA
   541  FRYKENSKVD DKWEKKCQKI FSFAHQTISA LIKAELAELP LRLFLQGALA AGEIGFENHE
   601  TVAYEFMSQA FSLYEDEISD SKAQLAAITL IIGTFERMKC FSEENHEPLR TQCALAASKL
   661  LKKPDQGRAV STCAHLFWSG RNTDKNGEEL HGGKRVMECL KKALKIANQC MDPSLQVQLF
   721  IEILNRYIYF YEKENDAVTI QVLNQLIQKI REDLPNLESS EETEQINKHF HNTLEHLRLR
   781  RESPESEGPI YEGLIL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against VPS35 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.25
Highest tissue expression
8.9 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 8.9 nTPM
  • parathyroid gland: 4.9 nTPM
  • retina: 3.7 nTPM
  • spleen: 3.7 nTPM
  • thymus: 3.6 nTPM
  • skeletal muscle: 3.5 nTPM

Single-cell type

  • early primary spermatocytes: 245 nCPM
  • respiratory ciliated cells: 220 nCPM
  • fallopian tube ciliated cells: 219 nCPM
  • esophageal apical cells: 211 nCPM
  • neutrophils: 209 nCPM
  • urothelial cells: 176 nCPM

Immune cell

  • non-classical monocyte: 2.3 nTPM
  • intermediate monocyte: 1.7 nTPM
  • basophil: 1.5 nTPM
  • myeloid DC: 1.5 nTPM
  • classical monocyte: 1.3 nTPM
  • eosinophil: 1.3 nTPM

Brain region

  • pons: 9.7 nTPM
  • white matter: 9.7 nTPM
  • cerebral cortex: 9.6 nTPM
  • basal ganglia: 9.3 nTPM
  • hippocampal formation: 9.2 nTPM
  • amygdala: 8.9 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about VPS35.

Disease | AllUniProt

Conditions VPS35 is implicated in, by any mechanism.

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 287 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.31
gnomAD pLI
0.97
gnomAD missense Z
3.53
DepMap mean gene effect
-0.59
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Armadillo-type fold
  • Vacuolar protein sorting-associated protein 35
  • Vacuolar protein sorting-associated protein 35, C-terminal
  • Vacuolar protein sorting-associated protein 35

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of VPS35 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads VPS35 as an antibody target. Whether an autoantibody or antibody against VPS35 could matter depends on whether native VPS35 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

VPS35 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label VPS35 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/VPS35. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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