Seroatlas · Human Serome Atlas

ANKRD50

Ankyrin repeat domain-containing protein 50

Also known as: ANR50_HUMAN, KIAA1223

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9ULJ7
Gene: ANKRD50
Ensembl: ENSG00000151458
Chromosome: 4
Canonical length: 1429 aa
Protein class: Predicted intracellular proteins, Transporters
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Involved in endocytic recycling. Predicted to be located in endosome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1429 residues, UniProt reviewed canonical sequence.

>Q9ULJ7|ANKRD50
     1  MTNPWEEKVC KMAQTSLLQG KQFYCREWVF HKLQHCLQEK SNCCNSAVNA PSLVMNSGNN
    61  ASGVSGKGAA WGVLLVGGPG SGKTALCTEL LWPSSPASLQ RGLHRQALAF HFCKAQDSDT
   121  LCVGGFIRGL VAQICRSGLL QGYEDKLRDP AVQSLLQPGE CERNPAEAFK RCVLLPLLGM
   181  KPPQQSLYLL VDSVDEGCNI TEGEQTSTSL SGTVAALLAG HHEFFPPWLL LLCSARKQSK
   241  AVTKMFTGFR KISLDDLRKA YIVKDVQQYI LHRLDQEEAL RQHLTKETAE MLNQLHIKSS
   301  GCFLYLERVL DGVVENFIML REIRDIPGTL NGLYLWLCQR LFVRKQFAKV QPILNVILAA
   361  CRPLTITELY HAVWTKNMSL TLEDFQRKLD ILSKLLVDGL GNTKILFHYS FAEWLLDVKH
   421  CTQKYLCNAA EGHRMLAMSY TCQAKNLTPL EAQEFALHLI NSNLQLETAE LALWMIWNGT
   481  PVRDSLSTLI PKEQEVLQLL VKAGAHVNSE DDRTSCIVRQ ALEREDSIRT LLDNGASVNQ
   541  CDSNGRTLLA NAAYSGSLDV VNLLVSRGAD LEIEDAHGHT PLTLAARQGH TKVVNCLIGC
   601  GANINHTDQD GWTALRSAAW GGHTEVVSAL LYAGVKVDCA DADSRTALRA AAWGGHEDIV
   661  LNLLQHGAEV NKADNEGRTA LIAAAYMGHR EIVEHLLDHG AEVNHEDVDG RTALSVAALC
   721  VPASKGHASV VSLLIDRGAE VDHCDKDGMT PLLVAAYEGH VDVVDLLLEG GADVDHTDNN
   781  GRTPLLAAAS MGHASVVNTL LFWGAAVDSI DSEGRTVLSI ASAQGNVEVV RTLLDRGLDE
   841  NHRDDAGWTP LHMAAFEGHR LICEALIEQG ARTNEIDNDG RIPFILASQE GHYDCVQILL
   901  ENKSNIDQRG YDGRNALRVA ALEGHRDIVE LLFSHGADVN CKDADGRPTL YILALENQLT
   961  MAEYFLENGA NVEASDAEGR TALHVSCWQG HMEMVQVLIA YHADVNAADN EKRSALQSAA
  1021  WQGHVKVVQL LIEHGAVVDH TCNQGATALC IAAQEGHIDV VQVLLEHGAD PNHADQFGRT
  1081  AMRVAAKNGH SQIIKLLEKY GASSLNGCSP SPVHTMEQKP LQSLSSKVQS LTIKSNSSGS
  1141  TGGGDMQPSL RGLPNGPTHA FSSPSESPDS TVDRQKSSLS NNSLKSSKNS SLRTTSSTAT
  1201  AQTVPIDSFH NLSFTEQIQQ HSLPRSRSRQ SIVSPSSTTQ SLGQSHNSPS SEFEWSQVKP
  1261  SLKSTKASKG GKSENSAKSG SAGKKAKQSN SSQPKVLEYE MTQFDRRGPI AKSGTAAPPK
  1321  QMPAESQCKI MIPSAQQEIG RSQQQFLIHQ QSGEQKKRNG IMTNPNYHLQ SNQVFLGRVS
  1381  VPRTMQDRGH QEVLEGYPSS ETELSLKQAL KLQIEGSDPS FNYKKETPL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKRD50 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 14 nTPM

Expression across tissuesHPA

Tissue

testis: 14 nTPM
placenta: 14 nTPM
urinary bladder: 13 nTPM
cervix: 11 nTPM
skin: 10 nTPM
lung: 9 nTPM

Single-cell type

late spermatids: 92 nCPM
prostatic glandular cells: 83 nCPM
urothelial cells: 82 nCPM
extravillous trophoblasts: 68 nCPM
basal keratinocytes: 56 nCPM
schwann cells: 54 nCPM

Immune cell

intermediate monocyte: 1.1 nTPM
myeloid DC: 0.8 nTPM
non-classical monocyte: 0.8 nTPM
classical monocyte: 0.5 nTPM
plasmacytoid DC: 0.3 nTPM
eosinophil: 0.2 nTPM

Brain region

hypothalamus: 22 nTPM
medulla oblongata: 16 nTPM
basal ganglia: 16 nTPM
midbrain: 16 nTPM
pons: 15 nTPM
cerebral cortex: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.31
gnomAD pLI: 0.98
gnomAD missense Z: 0.7
DepMap mean gene effect: 0.07
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

endosome

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ANKRD50 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

VPS35

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANKRD50 as an antibody target. Whether an autoantibody or antibody against ANKRD50 could matter depends on whether native ANKRD50 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANKRD50 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ANKRD50 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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