Seroatlas · Human Serome Atlas

WASHC2A

WASH complex subunit 2A

Also known as: bA56A21.1, bA98I6.1, FAM21A, FAM21B, FLJ10824, WAC2A_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q641Q2
Gene: WASHC2A
Ensembl: ENSG00000099290
Chromosome: 10
Canonical length: 1341 aa
Protein class: Predicted intracellular proteins, Transporters
Subcellular location: Nucleoli,Vesicles,Cytosol

OverviewNCBI Gene

Predicted to enable phosphatidylinositol phosphate binding activity and retromer complex binding activity. Involved in protein localization to endosome. Located in cytosol and nucleolus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1341 residues, UniProt reviewed canonical sequence.

>Q641Q2|WASHC2A
     1  MMNRTTPDQE LAPASEPVWE RPWSVEEIRR SSQSWSLAAD AGLLQFLQEF SQQTISRTHE
    61  IKKQVDGLIR ETKATDCRLH NVFNDFLMLS NTQFIENRVY DEEVEEPVLK AEAEKTEQEK
   121  TREQKEVDLI PKVQEAVNYG LQVLDSAFEQ LDIKAGNSDS EEDDANGRVE LILEPKDLYI
   181  DRPLPYLIGS KLFMEQEDVG LGELSSEEGS VGSDRGSIVD TEEEKEEEES DEDFAHHSDN
   241  EQNRHTTQMS DEEEDDDGCD LFADSEKEEE DIEDIEENTR PKRSRPTSFA DELAARIKGD
   301  AVGRVDEEPT TLPSGEAKPR KTLKEKKERR TPSDDEEDNL FAPPKLTDED FSPFGSGGGL
   361  FSGGKGLFDD EDEESDLFTE APQDRQAGAS VKEESSSSKP GKKIPAGAVS VFLGDTDVFG
   421  AASVPSMKEP QKPEQPTPRK SPYGPPPTGL FDDDDGDDDD DFFSAPHSKP SKTGKVQSTA
   481  DIFGDEEGDL FKEKAVASPE ATVSQTDENK ARAEKKVTLS SSKNLKPSSE TKTQKGLFSD
   541  EEDSEDLFSS QSASKLKGAS LLPGKLPTLV SLFDDEDEED NLFGGTAAKK QTLCLQAQRE
   601  EKAKASELSK KKASALLFSS DEEDQWNIPA SQTHLASDSR SKGEPRDSGT LQSQEAKAVK
   661  KTSLFEEDEE DDLFAIAKDS QKKTQRVSLL FEDDVDSGGS LFGSPPTSVP PATKKKETVS
   721  EAPPLLFSDE EEKEAQLGVK SVDKKVESAK ESLKFGRTDV AESEKEGLLT RSAQETVKHS
   781  DLFSSSSPWD KGTKPRTKTV LSLFDEEEDK MEDQNIIQAP QKEVGKGRDP DAHPKSTGVF
   841  QDEELLFSHK LQKDNDPDVD LFAGTKKTKL LEPSVGSLFG DDEDDDLFSS AKSQPLVQEK
   901  KRVVKKDHSV DSFKNQKHPE SIQGSKEKGI WKPETPQDSS GLAPFKTKEP STRIGKIQAN
   961  LAINPAALLP TAASQISEVK PVLPELAFPS SEHRRSHGLE SVPVLPGSGE AGVSFDLPAQ
  1021  ADTLHSANKS RVKMRGKRRP QTRAARRLAA QESSETEDMS VPRGPIAQWA DGAISPNGHR
  1081  PQLRAASGED STEEALAAAA APWEGGPVPG VDRSPFAKSL GHSRGEADLF DSGDIFSTGT
  1141  GSQSVERTKP KAKIAENPAN PPVGGKAKSP MFPALGEASS DDDLFQSAKP KPAKKTNPFP
  1201  LLEDEDDLFT DQKVKKNETK SNSQQDVILT TQDIFEDDIF ATEAIKPSQK TREKEKTLES
  1261  NLFDDNIDIF ADLTVKPKEK SKKKVEAKSI FDDDMDDIFS SGIQAKTTKP KSRSAQAAPE
  1321  PRFEHKVSNI FDDPLNAFGG Q

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against WASHC2A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.67
Highest tissue expression: 56 nTPM

Expression across tissuesHPA

Tissue

heart muscle: 56 nTPM
bone marrow: 36 nTPM
small intestine: 28 nTPM
cerebellum: 28 nTPM
colon: 27 nTPM
rectum: 27 nTPM

Single-cell type

respiratory ciliated cells: 67 nCPM
endometrial ciliated cells: 66 nCPM
retinal pigment epithelial cells: 60 nCPM
macrophages: 48 nCPM
prostatic glandular cells: 47 nCPM
monocytes: 46 nCPM

Immune cell

intermediate monocyte: 12 nTPM
MAIT T-cell: 10 nTPM
eosinophil: 10 nTPM
T-reg: 9.3 nTPM
gdT-cell: 9.1 nTPM
classical monocyte: 9 nTPM

Brain region

cerebellum: 39 nTPM
white matter: 38 nTPM
cerebral cortex: 38 nTPM
thalamus: 37 nTPM
pons: 35 nTPM
midbrain: 34 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.76
gnomAD pLI: 0
DepMap mean gene effect: -0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of WASHC2A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads WASHC2A as an antibody target. Whether an autoantibody or antibody against WASHC2A could matter depends on whether native WASHC2A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

WASHC2A is annotated at the cell surface, where native WASHC2A is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label WASHC2A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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