Seroatlas · Human Serome Atlas

ANKFY1

Rabankyrin-5

Also known as: ANFY1_HUMAN, ANKHZN, BTBD23, KIAA1255, RANK-5, ZFYVE14

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9P2R3
Gene
ANKFY1
Ensembl
ENSG00000185722
Chromosome
17
Canonical length
1169 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Endosomes

OverviewNCBI Gene

This gene encodes a cytoplasmic protein that contains a coiled-coil structure and a BTB/POZ domain at its N-terminus, ankyrin repeats in the middle portion, and a FYVE-finger motif at its C-terminus. This protein belongs to a subgroup of double zinc finger proteins which may be involved in vesicle or protein transport. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Apr 2012]

Canonical amino-acid sequenceUniProt

1169 residues, UniProt reviewed canonical sequence.

>Q9P2R3|ANKFY1
     1  MAEEEVAKLE KHLMLLRQEY VKLQKKLAET EKRCALLAAQ ANKESSSESF ISRLLAIVAD
    61  LYEQEQYSDL KIKVGDRHIS AHKFVLAARS DSWSLANLSS TKELDLSDAN PEVTMTMLRW
   121  IYTDELEFRE DDVFLTELMK LANRFQLQLL RERCEKGVMS LVNVRNCIRF YQTAEELNAS
   181  TLMNYCAEII ASHWDDLRKE DFSSMSAQLL YKMIKSKTEY PLHKAIKVER EDVVFLYLIE
   241  MDSQLPGKLN EADHNGDLAL DLALSRRLES IATTLVSHKA DVDMVDKSGW SLLHKGIQRG
   301  DLFAATFLIK NGAFVNAATL GAQETPLHLV ALYSSKKHSA DVMSEMAQIA EALLQAGANP
   361  NMQDSKGRTP LHVSIMAGNE YVFSQLLQCK QLDLELKDHE GSTALWLAVQ HITVSSDQSV
   421  NPFEDVPVVN GTSFDENSFA ARLIQRGSHT DAPDTATGNC LLQRAAGAGN EAAALFLATN
   481  GAHVNHRNKW GETPLHTACR HGLANLTAEL LQQGANPNLQ TEEALPLPKE AASLTSLADS
   541  VHLQTPLHMA IAYNHPDVVS VILEQKANAL HATNNLQIIP DFSLKDSRDQ TVLGLALWTG
   601  MHTIAAQLLG SGAAINDTMS DGQTLLHMAI QRQDSKSALF LLEHQADINV RTQDGETALQ
   661  LAIRNQLPLV VDAICTRGAD MSVPDEKGNP PLWLALANNL EDIASTLVRH GCDATCWGPG
   721  PGGCLQTLLH RAIDENNEPT ACFLIRSGCD VNSPRQPGAN GEGEEEARDG QTPLHLAASW
   781  GLEETVQCLL EFGANVNAQD AEGRTPIHVA ISSQHGVIIQ LLVSHPDIHL NVRDRQGLTP
   841  FACAMTFKNN KSAEAILKRE SGAAEQVDNK GRNFLHVAVQ NSDIESVLFL ISVHANVNSR
   901  VQDASKLTPL HLAVQAGSEI IVRNLLLAGA KVNELTKHRQ TALHLAAQQD LPTICSVLLE
   961  NGVDFAAVDE NGNNALHLAV MHGRLNNIRV LLTECTVDAE AFNLRGQSPL HILGQYGKEN
  1021  AAAIFDLFLE CMPGYPLDKP DADGSTVLLL AYMKGNANLC RAIVRSGARL GVNNNQGVNI
  1081  FNYQVATKQL LFRLLDMLSK EPPWCDGSYC YECTARFGVT TRKHHCRHCG RLLCHKCSTK
  1141  EIPIIKFDLN KPVRVCNICF DVLTLGGVS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKFY1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.27
Highest tissue expression
25 nTPM

Expression across tissuesHPA

Tissue

  • colon: 25 nTPM
  • cerebral cortex: 20 nTPM
  • thymus: 19 nTPM
  • skin: 19 nTPM
  • parathyroid gland: 18 nTPM
  • endometrium: 17 nTPM

Single-cell type

  • thymic myoid cells: 238 nCPM
  • retinal ganglion cells: 158 nCPM
  • myonuclei: 146 nCPM
  • neutrophil progenitors: 136 nCPM
  • corticotrophs: 108 nCPM
  • microglia: 105 nCPM

Immune cell

  • non-classical monocyte: 7.4 nTPM
  • intermediate monocyte: 6.4 nTPM
  • classical monocyte: 3.9 nTPM
  • myeloid DC: 2.8 nTPM
  • gdT-cell: 2.7 nTPM
  • MAIT T-cell: 2.5 nTPM

Brain region

  • medulla oblongata: 61 nTPM
  • white matter: 59 nTPM
  • spinal cord: 56 nTPM
  • basal ganglia: 54 nTPM
  • hypothalamus: 53 nTPM
  • midbrain: 52 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.31
gnomAD pLI
0.96
gnomAD missense Z
2.46
DepMap mean gene effect
-0.15
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ANKFY1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANKFY1 as an antibody target. Whether an autoantibody or antibody against ANKFY1 could matter depends on whether native ANKFY1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANKFY1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ANKFY1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ANKFY1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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