Seroatlas · Human Serome Atlas

NEK2

Serine/threonine-protein kinase Nek2

Also known as: NEK2_HUMAN, NEK2A, NLK1, PPP1R111, RP67

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P51955
Gene
NEK2
Ensembl
ENSG00000117650
Chromosome
1
Canonical length
445 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytokinetic bridge,Primary cilium,Centrosome,Basal body

OverviewNCBI Gene

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]

Canonical amino-acid sequenceUniProt

445 residues, UniProt reviewed canonical sequence.

>P51955|NEK2
     1  MPSRAEDYEV LYTIGTGSYG RCQKIRRKSD GKILVWKELD YGSMTEAEKQ MLVSEVNLLR
    61  ELKHPNIVRY YDRIIDRTNT TLYIVMEYCE GGDLASVITK GTKERQYLDE EFVLRVMTQL
   121  TLALKECHRR SDGGHTVLHR DLKPANVFLD GKQNVKLGDF GLARILNHDT SFAKTFVGTP
   181  YYMSPEQMNR MSYNEKSDIW SLGCLLYELC ALMPPFTAFS QKELAGKIRE GKFRRIPYRY
   241  SDELNEIITR MLNLKDYHRP SVEEILENPL IADLVADEQR RNLERRGRQL GEPEKSQDSS
   301  PVLSELKLKE IQLQERERAL KAREERLEQK EQELCVRERL AEDKLARAEN LLKNYSLLKE
   361  RKFLSLASNP ELLNLPSSVI KKKVHFSGES KENIMRSENS ESQLTSKSKC KDLKKRLHAA
   421  QLRAQALSDI EKNYQLKSRQ ILGMR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NEK2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
63 nTPM

Expression across tissuesHPA

Tissue

  • testis: 63 nTPM
  • thymus: 18 nTPM
  • bone marrow: 17 nTPM
  • tonsil: 9.9 nTPM
  • lymph node: 8.4 nTPM
  • rectum: 7.2 nTPM

Single-cell type

  • late primary spermatocytes: 414 nCPM
  • early primary spermatocytes: 100 nCPM
  • gastric progenitor cells: 60 nCPM
  • monocyte progenitors: 53 nCPM
  • early spermatids: 52 nCPM
  • late spermatids: 43 nCPM

Immune cell

  • T-reg: 1.6 nTPM
  • NK-cell: 1.5 nTPM
  • basophil: 1.1 nTPM
  • neutrophil: 0.6 nTPM
  • memory B-cell: 0.4 nTPM
  • naive B-cell: 0.4 nTPM

Brain region

  • white matter: 4 nTPM
  • cerebellum: 3.9 nTPM
  • thalamus: 3.7 nTPM
  • choroid plexus: 3.6 nTPM
  • cerebral cortex: 3.4 nTPM
  • hippocampal formation: 3.4 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NEK2.

Disease | AllUniProt

Conditions NEK2 is implicated in, by any mechanism.

Disease | GeneticClinVar

5 pathogenic / likely-pathogenic of 300 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.82
gnomAD pLI
0
gnomAD missense Z
1.3
DepMap mean gene effect
0.04
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NEK2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NEK2 as an antibody target. Whether an autoantibody or antibody against NEK2 could matter depends on whether native NEK2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NEK2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NEK2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NEK2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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