Seroatlas · Human Serome Atlas

ATF4

Cyclic AMP-dependent transcription factor ATF-4

Also known as: ATF4_HUMAN, CREB-2, TAXREB67, TXREB

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P18848
Gene: ATF4
Ensembl: ENSG00000128272
Chromosome: 22
Canonical length: 351 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm
Quaternary structure: Homodimer

OverviewNCBI Gene

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]

Canonical amino-acid sequenceUniProt

351 residues, UniProt reviewed canonical sequence.

>P18848|ATF4
     1  MTEMSFLSSE VLVGDLMSPF DQSGLGAEES LGLLDDYLEV AKHFKPHGFS SDKAKAGSSE
    61  WLAVDGLVSP SNNSKEDAFS GTDWMLEKMD LKEFDLDALL GIDDLETMPD DLLTTLDDTC
   121  DLFAPLVQET NKQPPQTVNP IGHLPESLTK PDQVAPFTFL QPLPLSPGVL SSTPDHSFSL
   181  ELGSEVDITE GDRKPDYTAY VAMIPQCIKE EDTPSDNDSG ICMSPESYLG SPQHSPSTRG
   241  SPNRSLPSPG VLCGSARPKP YDPPGEKMVA AKVKGEKLDK KLKKMEQNKT AATRYRQKKR
   301  AEQEALTGEC KELEKKNEAL KERADSLAKE IQYLKDLIEE VRKARGKKRV P

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ATF4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.66
Highest tissue expression: 837 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 837 nTPM
colon: 826 nTPM
skeletal muscle: 771 nTPM
blood vessel: 703 nTPM
pancreas: 621 nTPM
urinary bladder: 580 nTPM

Single-cell type

epididymal basal cells: 615 nCPM
esophageal apical cells: 600 nCPM
platelets: 502 nCPM
breast secretory cells: 416 nCPM
extravillous trophoblasts: 375 nCPM
goblet cells: 319 nCPM

Immune cell

basophil: 47 nTPM
myeloid DC: 45 nTPM
memory B-cell: 38 nTPM
classical monocyte: 37 nTPM
non-classical monocyte: 35 nTPM
T-reg: 32 nTPM

Brain region

white matter: 233 nTPM
medulla oblongata: 218 nTPM
cerebellum: 209 nTPM
thalamus: 189 nTPM
pons: 189 nTPM
spinal cord: 185 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.09
gnomAD pLI: 0.02
gnomAD missense Z: -3.54
DepMap mean gene effect: -0.44
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ATF4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ATF4 as an antibody target. Whether an autoantibody or antibody against ATF4 could matter depends on whether native ATF4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ATF4 is annotated at the cell surface, where native ATF4 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ATF4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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