Seroatlas · Human Serome Atlas

NSL1

Kinetochore-associated protein NSL1 homolog

Also known as: C1orf48, DC8, DKFZP566O1646, MIS14, NSL1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96IY1
Gene
NSL1
Ensembl
ENSG00000117697
Chromosome
1
Canonical length
281 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nuclear speckles

OverviewNCBI Gene

This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. There is a pseudogene of the 3' UTR region of this gene on chromosome X. [provided by RefSeq, Jul 2014]

Canonical amino-acid sequenceUniProt

281 residues, UniProt reviewed canonical sequence.

>Q96IY1|NSL1
     1  MAGSPELVVL DPPWDKELAA GTESQALVSA TPREDFRVRC TSKRAVTEML QLCGRFVQKL
    61  GDALPEEIRE PALRDAQWTF ESAVQENISI NGQAWQEASD NCFMDSDIKV LEDQFDEIIV
   121  DIATKRKQYP RKILECVIKT IKAKQEILKQ YHPVVHPLDL KYDPDPAPHM ENLKCRGETV
   181  AKEISEAMKS LPALIEQGEG FSQVLRMQPV IHLQRIHQEV FSSCHRKPDA KPENFITQIE
   241  TTPTETASRK TSDMVLKRKQ TKDCPQRKWY PLRPKKINLD T

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NSL1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.55
Highest tissue expression
26 nTPM

Expression across tissuesHPA

Tissue

  • spinal cord: 26 nTPM
  • lymph node: 25 nTPM
  • heart muscle: 23 nTPM
  • skeletal muscle: 21 nTPM
  • thymus: 21 nTPM
  • cerebral cortex: 19 nTPM

Single-cell type

  • kupffer cells: 158 nCPM
  • oocytes: 124 nCPM
  • microglia: 109 nCPM
  • hofbauer cells: 91 nCPM
  • oligodendrocytes: 85 nCPM
  • differentiating spermatogonia: 85 nCPM

Immune cell

  • non-classical monocyte: 65 nTPM
  • eosinophil: 63 nTPM
  • intermediate monocyte: 62 nTPM
  • total PBMC: 61 nTPM
  • memory B-cell: 59 nTPM
  • myeloid DC: 56 nTPM

Brain region

  • white matter: 35 nTPM
  • medulla oblongata: 29 nTPM
  • spinal cord: 28 nTPM
  • cerebellum: 27 nTPM
  • basal ganglia: 26 nTPM
  • pons: 26 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.89
gnomAD pLI
0
gnomAD missense Z
0.24
DepMap mean gene effect
-1.17
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Kinetochore Mis14/Nsl1
  • Kinetochore protein Mis14 like

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NSL1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NSL1 as an antibody target. Whether an autoantibody or antibody against NSL1 could matter depends on whether native NSL1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NSL1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NSL1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NSL1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...