Seroatlas · Human Serome Atlas

WASHC3

WASH complex subunit 3

Also known as: CCDC53, CGI-116, WASC3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y3C0
Gene: WASHC3
Ensembl: ENSG00000120860
Chromosome: 12
Canonical length: 194 aa
Protein class: Predicted intracellular proteins, Transporters
Subcellular location: Vesicles

OverviewNCBI Gene

Predicted to be involved in actin filament polymerization and exocytosis. Part of WASH complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

194 residues, UniProt reviewed canonical sequence.

>Q9Y3C0|WASHC3
     1  MDEDGLPLMG SGIDLTKVPA IQQKRTVAFL NQFVVHTVQF LNRFSTVCEE KLADLSLRIQ
    61  QIETTLNILD AKLSSIPGLD DVTVEVSPLN VTSVTNGAHP EATSEQPQQN STQDSGLQES
   121  EVSAENILTV AKDPRYARYL KMVQVGVPVM AIRNKMISEG LDPDLLERPD APVPDGESEK
   181  TVEESSDSES SFSD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against WASHC3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.56
Highest tissue expression: 96 nTPM

Expression across tissuesHPA

Tissue

testis: 96 nTPM
spinal cord: 54 nTPM
midbrain: 46 nTPM
amygdala: 44 nTPM
hypothalamus: 44 nTPM
basal ganglia: 42 nTPM

Single-cell type

late spermatids: 2,085 nCPM
early spermatids: 885 nCPM
late primary spermatocytes: 703 nCPM
early primary spermatocytes: 185 nCPM
esophageal apical cells: 142 nCPM
decidual stromal cells: 139 nCPM

Immune cell

basophil: 127 nTPM
T-reg: 119 nTPM
eosinophil: 96 nTPM
non-classical monocyte: 71 nTPM
intermediate monocyte: 67 nTPM
memory B-cell: 62 nTPM

Brain region

pons: 20 nTPM
hypothalamus: 20 nTPM
medulla oblongata: 19 nTPM
white matter: 18 nTPM
midbrain: 17 nTPM
spinal cord: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.1
gnomAD pLI: 0
DepMap mean gene effect: -0.21
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

WASH complex subunit 3
Subunit CCDC53 of WASH complex

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of WASHC3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads WASHC3 as an antibody target. Whether an autoantibody or antibody against WASHC3 could matter depends on whether native WASHC3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

WASHC3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label WASHC3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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