Seroatlas · Human Serome Atlas

ZWINT

Outer kinetochore KNL1 complex subunit ZWINT

Also known as: KNTC2AP, SIP30, ZWINT_HUMAN, Zwint1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O95229
Gene
ZWINT
Ensembl
ENSG00000122952
Chromosome
10
Canonical length
277 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear bodies,Cytosol

OverviewNCBI Gene

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

277 residues, UniProt reviewed canonical sequence.

>O95229|ZWINT
     1  MEAAETEAEA AALEVLAEVA GILEPVGLQE EAELPAKILV EFVVDSQKKD KLLCSQLQVA
    61  DFLQNILAQE DTAKGLDPLA SEDTSRQKAI AAKEQWKELK ATYREHVEAI KIGLTKALTQ
   121  MEEAQRKRTQ LREAFEQLQA KKQMAMEKRR AVQNQWQLQQ EKHLQHLAEV SAEVRERKTG
   181  TQQELDRVFQ KLGNLKQQAE QERDKLQRYQ TFLQLLYTLQ GKLLFPEAEA EAENLPDDKP
   241  QQPTRPQEQS TGDTMGRDPG VSFKAVGLQP AGDVNLP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZWINT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.53
Highest tissue expression
41 nTPM

Expression across tissuesHPA

Tissue

  • thymus: 41 nTPM
  • bone marrow: 39 nTPM
  • tonsil: 31 nTPM
  • lymph node: 25 nTPM
  • testis: 24 nTPM
  • rectum: 21 nTPM

Single-cell type

  • late spermatids: 301 nCPM
  • late primary spermatocytes: 142 nCPM
  • oocytes: 138 nCPM
  • early primary spermatocytes: 129 nCPM
  • extravillous trophoblasts: 123 nCPM
  • migrating cytotrophoblasts: 107 nCPM

Immune cell

  • T-reg: 17 nTPM
  • naive CD4 T-cell: 6.4 nTPM
  • memory CD4 T-cell: 5.3 nTPM
  • memory CD8 T-cell: 3.6 nTPM
  • naive CD8 T-cell: 3.6 nTPM
  • NK-cell: 3.5 nTPM

Brain region

  • thalamus: 2.2 nTPM
  • hippocampal formation: 1.8 nTPM
  • white matter: 1.7 nTPM
  • cerebral cortex: 1.5 nTPM
  • midbrain: 1.4 nTPM
  • hypothalamus: 1.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.31
gnomAD pLI
0
gnomAD missense Z
-0.5
DepMap mean gene effect
-0.42
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • ZW10 interactor
  • ZW10 interactor

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ZWINT in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZWINT as an antibody target. Whether an autoantibody or antibody against ZWINT could matter depends on whether native ZWINT is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZWINT is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZWINT as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ZWINT. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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