Seroatlas · Human Serome Atlas

RUFY4

RUN and FYVE domain-containing protein 4

Also known as: FLJ46536, RUFY4_HUMAN, ZFYVE31

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6ZNE9
Gene: RUFY4
Ensembl: ENSG00000188282
Chromosome: 2
Canonical length: 571 aa
Protein class: Predicted intracellular proteins
Quaternary structure: Homodimer

OverviewNCBI Gene

Enables phosphatidylinositol-3-phosphate binding activity. Involved in autophagosome assembly; cellular response to interleukin-4; and positive regulation of macroautophagy. Located in autophagosome. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

571 residues, UniProt reviewed canonical sequence.

>Q6ZNE9|RUFY4
     1  MAEEGAILKV TKDLRAAVSA ILQGYGDGQG PVTDTSAELH RLCGCLELLL QFDQKEQKSF
    61  LGPRKDYWDF LCTALRRQRG NMEPIHFVRS QDKLKTPLGK GRAFIRFCLA RGQLAEALQL
   121  CLLNSELTRE WYGPRSPLLC PERQEDILDS LYALNGVAFE LDLQQPDLDG AWPMFSESRC
   181  SSSTQTQGRR PRKNKDAPKK IPAAYGGPEN VQIEDSHTSQ AICLQDAPSG QQLAGLPRSQ
   241  QQRHLPFFLE KKGESSRKHR YPQSMWEPEG KELQLDQEER APWIEIFLGN STPSTQGQGK
   301  GAMGTQKEVI GMEAEVTGVL LVAEGQRTTE GTHKKEAEWS HVQRLLMPSP RGAVEGAVSG
   361  SRQGSGGSSI LGEPWVLQGH ATKEDSTVEN PQVQTEVTLV ARREEQAEVS LQDEIKSLRL
   421  GLRKAEEQAQ RQEQLLREQE GELQALREQL SRCQEERAEL QAQLEQKQQE AERRDAMYQE
   481  ELGGQRDLVQ AMKRRVLELI QEKDRLWQRL QHLSSMAPEC CVACSKIFGR FSRRYPCRLC
   541  GGLLCHACSM DYKKRDRCCP PCAQGREAQV T

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RUFY4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.49
Highest tissue expression: 2.1 nTPM

Expression across tissuesHPA

Tissue

spleen: 2.1 nTPM
thymus: 1.6 nTPM
hippocampal formation: 1.4 nTPM
prostate: 1.2 nTPM
lymph node: 1.1 nTPM
appendix: 0.9 nTPM

Single-cell type

kupffer cells: 33 nCPM
oligodendrocyte progenitor cells: 16 nCPM
megakaryocytes: 12 nCPM
platelets: 9.8 nCPM
pdcs: 9.2 nCPM
prostatic hillock cells: 7.9 nCPM

Immune cell

myeloid DC: 0.2 nTPM
non-classical monocyte: 0.1 nTPM
plasmacytoid DC: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM

Brain region

cerebral cortex: 2.9 nTPM
hippocampal formation: 1.2 nTPM
spinal cord: 0.4 nTPM
amygdala: 0.2 nTPM
thalamus: 0.2 nTPM
white matter: 0.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.19
gnomAD pLI: 0
gnomAD missense Z: -0.07

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

RUN domain
Zinc finger, FYVE/PHD-type
Zinc finger, RING/FYVE/PHD-type
Zinc finger, FYVE-related
RUN domain superfamily
RUN domain
RUN and FYVE domain-containing protein 4
RUFY4-like domain
RUFY4-like domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RUFY4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RUFY4 as an antibody target. Whether an autoantibody or antibody against RUFY4 could matter depends on whether native RUFY4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RUFY4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RUFY4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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