Seroatlas · Human Serome Atlas

CENPH

Centromere protein H

Also known as: CENPH_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H3R5
Gene: CENPH
Ensembl: ENSG00000153044
Chromosome: 5
Canonical length: 247 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli rim,Mitotic chromosome

OverviewNCBI Gene

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

247 residues, UniProt reviewed canonical sequence.

>Q9H3R5|CENPH
     1  MEEQPQMQDA DEPADSGGEG RAGGPPQVAG AQAACSEDRM TLLLRLRAQT KQQLLEYKSM
    61  VDASEEKTPE QIMQEKQIEA KIEDLENEIE EVKVAFEIKK LALDRMRLST ALKKNLEKIS
   121  RQSSVLMDNM KHLLELNKLI MKSQQESWDL EEKLLDIRKK RLQLKQASES KLLEIQTEKN
   181  KQKIDLDSME NSERIKIIRQ NLQMEIKITT VIQHVFQNLI LGSKVNWAED PALKEIVLQL
   241  EKNVDMM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CENPH can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.51
Highest tissue expression: 32 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 32 nTPM
testis: 28 nTPM
tonsil: 18 nTPM
thymus: 17 nTPM
lymph node: 17 nTPM
skin: 8.7 nTPM

Single-cell type

early primary spermatocytes: 308 nCPM
oocytes: 290 nCPM
differentiating spermatogonia: 222 nCPM
erythrocyte progenitors: 123 nCPM
undifferentiated spermatogonia: 75 nCPM
migrating cytotrophoblasts: 61 nCPM

Immune cell

naive B-cell: 14 nTPM
memory B-cell: 13 nTPM
naive CD4 T-cell: 9.9 nTPM
T-reg: 9.8 nTPM
naive CD8 T-cell: 8.6 nTPM
NK-cell: 8.6 nTPM

Brain region

white matter: 4.4 nTPM
basal ganglia: 3.5 nTPM
cerebellum: 3.5 nTPM
cerebral cortex: 3.5 nTPM
medulla oblongata: 3.5 nTPM
pons: 3.4 nTPM

ReferencesPubMed · IEDB

Publications for CENPH from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

3 publications

Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere--kinetochore complexes.
2000 · Hum Mol Genet · RCR 1.4 · 89 citations
Anti-CENP-H antibodies in patients with Sjogren's syndrome.
2006 · Rheumatol Int · RCR 0.8 · 30 citations
Low prevalence of autoantibodies to CENP-H, -I, -K, -L, -M, -N, -T and -U in a Japanese cohort of anti-centromere positive samples.
2013 · Immunopharmacol Immunotoxicol · RCR 0.1 · 4 citations

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.14
gnomAD pLI: 0
gnomAD missense Z: 0.64
DepMap mean gene effect: -0.76
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

kinetochore binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

Centromere protein H, C-terminal
Centromere protein H
Centromere protein H (CENP-H)

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CENPH in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CENPH as an antibody target. Whether an autoantibody or antibody against CENPH could matter depends on whether native CENPH is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CENPH is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CENPH as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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