Seroatlas · Human Serome Atlas

SPC25

Kinetochore protein Spc25

Also known as: AD024, MGC22228, SPBC25, SPC25_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9HBM1
Gene: SPC25
Ensembl: ENSG00000152253
Chromosome: 2
Canonical length: 224 aa
Protein class: Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

224 residues, UniProt reviewed canonical sequence.

>Q9HBM1|SPC25
     1  MVEDELALFD KSINEFWNKF KSTDTSCQMA GLRDTYKDSI KAFAEKLSVK LKEEERMVEM
    61  FLEYQNQISR QNKLIQEKKD NLLKLIAEVK GKKQELEVLT ANIQDLKEEY SRKKETISTA
   121  NKANAERLKR LQKSADLYKD RLGLEIRKIY GEKLQFIFTN IDPKNPESPF MFSLHLNEAR
   181  DYEVSDSAPH LEGLAEFQEN VRKTNNFSAF LANVRKAFTA TVYN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPC25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.41
Highest tissue expression: 8 nTPM

Expression across tissuesHPA

Tissue

thymus: 8 nTPM
esophagus: 4.2 nTPM
bone marrow: 3.8 nTPM
lymph node: 3.7 nTPM
tonsil: 3.6 nTPM
testis: 3.2 nTPM

Single-cell type

oocytes: 198 nCPM
oligodendrocyte progenitor cells: 65 nCPM
megakaryocyte progenitors: 28 nCPM
erythrocyte progenitors: 22 nCPM
migrating cytotrophoblasts: 22 nCPM
extravillous trophoblasts: 21 nCPM

Immune cell

T-reg: 0.6 nTPM
neutrophil: 0.3 nTPM
memory B-cell: 0.2 nTPM
memory CD4 T-cell: 0.2 nTPM
naive B-cell: 0.2 nTPM
eosinophil: 0.1 nTPM

Brain region

hippocampal formation: 5.4 nTPM
white matter: 5.2 nTPM
cerebral cortex: 5 nTPM
pons: 4.5 nTPM
hypothalamus: 4 nTPM
midbrain: 3.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.76
gnomAD pLI: 0.03
gnomAD missense Z: 0.68
DepMap mean gene effect: -1.67
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

Chromosome segregation protein Spc25, C-terminal
Kinetochore protein Spc25
Chromosome segregation protein Spc25

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SPC25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPC25 as an antibody target. Whether an autoantibody or antibody against SPC25 could matter depends on whether native SPC25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPC25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPC25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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